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Literature summary extracted from

  • Setoyama, C.; Nishina,Y.; Tamaoki, H.; Mizutani, H.; Miyahara, I.; Hirotsu, K.; Shiga, K.; Miura, R.
    Effects of hydrogen bond in association with flavin and substrate in flavoenzyme D-amino acid oxidase. The catalytic and structural roles of gly313 and thr317 (2002), J. Biochem., 131, 59-69.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 G313A decreased activities to various D-amino acids Sus scrofa
1.4.3.3 T317A decreased activity to FAD Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.67
-
 D-valine wild-type Sus scrofa
1.4.3.3 1
-
 D-alanine mutant T317A Sus scrofa
1.4.3.3 1.1
-
 D-alanine wild-type Sus scrofa
1.4.3.3 1.1
-
 D-proline wild-type Sus scrofa
1.4.3.3 1.2
-
 D-proline mutant T317A Sus scrofa
1.4.3.3 1.4
-
 D-phenylalanine wild-type Sus scrofa
1.4.3.3 4.3
-
 D-valine mutant T317A Sus scrofa
1.4.3.3 4.8
-
 D-serine wild-type Sus scrofa
1.4.3.3 9.1
-
 D-phenylalanine mutant T317A Sus scrofa
1.4.3.3 20.8
-
 D-serine mutant T317A Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-serine + H2O + O2 Sus scrofa
-
 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-serine + H2O + O2
-
 Sus scrofa 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2
-
 Sus scrofa 3-hydroxypyruvate + NH3 + H2O2
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 0.9
-
 D-valine mutant T317A Sus scrofa
1.4.3.3 0.92
-
 D-alanine mutant T317A Sus scrofa
1.4.3.3 1.8
-
 D-serine mutant T317A Sus scrofa
1.4.3.3 2.4
-
 D-phenylalanine mutant T317A Sus scrofa
1.4.3.3 2.5
-
 D-valine wild-type Sus scrofa
1.4.3.3 2.7
-
 D-serine wild-type Sus scrofa
1.4.3.3 8.5
-
 D-alanine wild-type Sus scrofa
1.4.3.3 9.7
-
 D-proline mutant T317A Sus scrofa
1.4.3.3 17
-
 D-phenylalanine wild-type Sus scrofa
1.4.3.3 21
-
 D-proline wild-type Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD flavoprotein Sus scrofa