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Literature summary extracted from

  • Simonetta, M.P.; Vanoni, M.A.; Casalin, P.
    Purification and properties of D-amino-acid oxidase, an inducible flavoenzyme from Rhodotorula gracilis (1987), Biochim. Biophys. Acta, 914, 136-142.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.4.3.3 cetylpyridinium bromide stabilizes Rhodotorula toruloides
1.4.3.3 glycerol, 10%, stabilizes Rhodotorula toruloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 37000
-
2 * 37000, SDS-PAGE Rhodotorula toruloides
1.4.3.3 79000
-
gel filtration Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2 Rhodotorula toruloides
-
pyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-asparagine + H2O + O2 Rhodotorula toruloides
-
2-oxosuccinamic acid + NH3 + H2O2
-
?
1.4.3.3 D-cysteine + H2O + O2 Rhodotorula toruloides
-
2-oxo-3-thiopropionic acid + NH3 + H2O2
-
?
1.4.3.3 D-glutamine + H2O + O2 Rhodotorula toruloides
-
2-oxoglutaramate + NH3 + H2O2
-
?
1.4.3.3 D-isoleucine + H2O + O2 Rhodotorula toruloides
-
3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-leucine + H2O + O2 Rhodotorula toruloides
-
4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-methionine + H2O + O2 Rhodotorula toruloides
-
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-phenylalanine + H2O + O2 Rhodotorula toruloides
-
phenylpyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-proline + H2O + O2 Rhodotorula toruloides
-
2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-serine + H2O + O2 Rhodotorula toruloides
-
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
?
1.4.3.3 D-threonine + H2O + O2 Rhodotorula toruloides
-
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
?
1.4.3.3 D-tryptophan + H2O + O2 Rhodotorula toruloides
-
indol-3-pyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-valine + H2O + O2 Rhodotorula toruloides
-
alpha-ketoisovaleric acid + NH3 + H2O2
-
?
1.4.3.3 phenylglycine + H2O + O2 Rhodotorula toruloides
-
benzoylformic acid + NH3 + H2O2
-
?
1.4.3.3 thiazolidine-2-carboxylic acid + H2O + O2 Rhodotorula toruloides
-
? + NH3 + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
Rhodotorula toruloides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 57.8
-
-
Rhodotorula toruloides

Storage Stability

EC Number Storage Stability Organism
1.4.3.3 -80°C, 2-3 months, stable Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
Rhodotorula toruloides pyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-asparagine + H2O + O2
-
Rhodotorula toruloides 2-oxosuccinamic acid + NH3 + H2O2
-
?
1.4.3.3 D-cysteine + H2O + O2
-
Rhodotorula toruloides 2-oxo-3-thiopropionic acid + NH3 + H2O2
-
?
1.4.3.3 D-glutamine + H2O + O2
-
Rhodotorula toruloides 2-oxoglutaramate + NH3 + H2O2
-
?
1.4.3.3 D-isoleucine + H2O + O2
-
Rhodotorula toruloides 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-leucine + H2O + O2
-
Rhodotorula toruloides 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-methionine + H2O + O2
-
Rhodotorula toruloides 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-phenylalanine + H2O + O2
-
Rhodotorula toruloides phenylpyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-proline + H2O + O2
-
Rhodotorula toruloides 2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-serine + H2O + O2
-
Rhodotorula toruloides 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
?
1.4.3.3 D-threonine + H2O + O2
-
Rhodotorula toruloides 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
?
1.4.3.3 D-tryptophan + H2O + O2
-
Rhodotorula toruloides indol-3-pyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-valine + H2O + O2
-
Rhodotorula toruloides alpha-ketoisovaleric acid + NH3 + H2O2
-
?
1.4.3.3 phenylglycine + H2O + O2
-
Rhodotorula toruloides benzoylformic acid + NH3 + H2O2
-
?
1.4.3.3 thiazolidine-2-carboxylic acid + H2O + O2
-
Rhodotorula toruloides ? + NH3 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 dimer 2 * 37000, SDS-PAGE Rhodotorula toruloides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.3 8
-
unstable above Rhodotorula toruloides

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD tightly bound Rhodotorula toruloides
1.4.3.3 FAD flavoprotein Rhodotorula toruloides