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Literature summary extracted from

  • Brearley, G.M.; Price, C.P.; Atkinson, T.; Hammond, P.M.
    Purification and partial characterization of a broad-range L-amino acid oxidase from Bacillus carotarum 2Pfa isolated from soil (1994), Appl. Microbiol. Biotechnol., 41, 670-676.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.037
-
 L-arginine
-
 Bacillus carotarum
1.4.3.2 0.259
-
 L-asparagine
-
 Bacillus carotarum
1.4.3.2 0.34
-
 L-glutamine
-
 Bacillus carotarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 54000
-
 2 * 54000, SDS-PAGE Bacillus carotarum
1.4.3.2 102000 115000 gel filtration, native PAGE Bacillus carotarum

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bacillus carotarum
-
 2Pfa
-
1.4.3.2 Bacillus carotarum 2Pfa
-
 2Pfa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2
-
 Bacillus carotarum

Storage Stability

EC Number Storage Stability Organism
1.4.3.2 -20°C, 4 months, no loss of activity Bacillus carotarum
1.4.3.2 4°C, 4 months, no loss of activity Bacillus carotarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-amino acid + H2O + O2
-
 Bacillus carotarum 2-oxo acid + NH3 + H2O2
-
 ?
1.4.3.2 L-amino acid + H2O + O2
-
 Bacillus carotarum 2Pfa 2-oxo acid + NH3 + H2O2
-
 ?
1.4.3.2 L-arginine + H2O + O2
-
 Bacillus carotarum 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-arginine + H2O + O2
-
 Bacillus carotarum 2Pfa 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-asparagine + H2O + O2
-
 Bacillus carotarum alpha-ketosuccinamic acid + NH3 + H2O2
-
 r
1.4.3.2 L-glutamine + H2O + O2
-
 Bacillus carotarum 2-oxoglutaramate + NH3 + H2O2
-
 r
1.4.3.2 L-leucine + H2O + O2
-
 Bacillus carotarum 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine + H2O + O2
-
 Bacillus carotarum 2Pfa 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-lysine + H2O + O2
-
 Bacillus carotarum 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-methionine + H2O + O2
-
 Bacillus carotarum 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-methionine + H2O + O2
-
 Bacillus carotarum 2Pfa 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2
-
 Bacillus carotarum phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2
-
 Bacillus carotarum 2Pfa phenylpyruvate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 dimer 2 * 54000, SDS-PAGE Bacillus carotarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 50
-
-
 Bacillus carotarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 8 8.5 L-phenylalanine Bacillus carotarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.2 4.5 10.5
-
 Bacillus carotarum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD 2 mol of FAD per mol of enzyme Bacillus carotarum