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Literature summary extracted from

  • Vallon, O.; Bulte, L.; Kuras, R.; Olive, J.; Wollman, F.A.
    Extensive accumulation of an extracellular L-amino acid oxidase during gametogenesis of Chlamydomonas reinhardtii (1993), Eur. J. Biochem., 215, 351-360.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.2 CaCl2 2 mM, 50% activation Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.2 EDTA 10 mM, 90% inhibition Chlamydomonas reinhardtii
1.4.3.2 hydroxylamine 5 mM, 75% inhibition Chlamydomonas reinhardtii
1.4.3.2 KCN 10 mM, complete inhibition Chlamydomonas reinhardtii
1.4.3.2 NaF 10 mM, 85% inhibition Chlamydomonas reinhardtii
1.4.3.2 Thiosemicarbazide 5 mM, 40% inhibition Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 2.2
-
 S-ribosyl-L-homocysteine
-
 Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.2 periplasm
-
 Chlamydomonas reinhardtii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 66000
-
 Malpha, ? * 66000, Mbeta, ? * 66000 + ? * 135000, latter polypeptide not required for amino acid oxidase activity Chlamydomonas reinhardtii
1.4.3.2 135000
-
 Malpha, ? * 66000, Mbeta, ? * 66000 + ? * 135000, latter polypeptide not required for amino acid oxidase activity Chlamydomonas reinhardtii
1.4.3.2 900000 1000000 form Malpha, gel filtration, sedimentation equilibrium Chlamydomonas reinhardtii
1.4.3.2 1200000 1300000 form Mbeta, gel filtration, sedimentation equilibrium Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 L-amino acid + H2O + O2 Chlamydomonas reinhardtii operates as scavanger of ammonium from extracellular amino acids 2-oxo acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Chlamydomonas reinhardtii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.2 glycoprotein both polypeptide chains are glycosylated Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2
-
 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.2 1.4
-
 glycine Chlamydomonas reinhardtii
1.4.3.2 4.4
-
 L-threonine Chlamydomonas reinhardtii
1.4.3.2 4.9
-
 L-aspartate Chlamydomonas reinhardtii
1.4.3.2 6
-
 L-glutamate Chlamydomonas reinhardtii
1.4.3.2 12.5
-
 L-histidine Chlamydomonas reinhardtii
1.4.3.2 14.4
-
 L-valine Chlamydomonas reinhardtii
1.4.3.2 14.8
-
 L-tyrosine Chlamydomonas reinhardtii
1.4.3.2 15.5
-
 L-tryptophan Chlamydomonas reinhardtii
1.4.3.2 16.6
-
 L-arginine Chlamydomonas reinhardtii
1.4.3.2 17
-
 L-isoleucine Chlamydomonas reinhardtii
1.4.3.2 17.4
-
 S-adenosy-L-cysteine Chlamydomonas reinhardtii
1.4.3.2 17.8
-
 L-alanine Chlamydomonas reinhardtii
1.4.3.2 18.4
-
 L-asparagine Chlamydomonas reinhardtii
1.4.3.2 18.8
-
 L-leucine Chlamydomonas reinhardtii
1.4.3.2 18.9
-
 L-lysine Chlamydomonas reinhardtii
1.4.3.2 20.4
-
 L-cystine Chlamydomonas reinhardtii
1.4.3.2 21.4
-
 L-ornithine Chlamydomonas reinhardtii
1.4.3.2 24.2
-
 L-serine Chlamydomonas reinhardtii
1.4.3.2 24.3
-
 L-methionine Chlamydomonas reinhardtii
1.4.3.2 25.2
-
 S-adenosyl-L-homocysteine Chlamydomonas reinhardtii
1.4.3.2 26.5
-
 L-phenylalanine Chlamydomonas reinhardtii
1.4.3.2 26.5
-
 L-ethionine Chlamydomonas reinhardtii
1.4.3.2 30.5
-
 L-methionine sulfoximine Chlamydomonas reinhardtii
1.4.3.2 39.5
-
 L-glutamine Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-amino acid + H2O + O2 all amino acids except cysteine Chlamydomonas reinhardtii 2-oxo acid + NH3 + H2O2
-
 ?
1.4.3.2 L-amino acid + H2O + O2 operates as scavanger of ammonium from extracellular amino acids Chlamydomonas reinhardtii 2-oxo acid + NH3 + H2O2
-
 ?
1.4.3.2 S-ribosyl-L-homocysteine + H2O + O2
-
 Chlamydomonas reinhardtii 4-thioribosyl-2-oxobutyrate + NH3 + H2O2
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.3.2 oligomer Malpha, ? * 66000, Mbeta, ? * 66000 + ? * 135000, latter polypeptide not required for amino acid oxidase activity Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 9
-
 broad optimum Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD non-covalently bound Chlamydomonas reinhardtii