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Literature summary extracted from

  • Ueda, M.; Chang, C.C.; Ohno, M.
    Purification and characterization of L-amino acid oxidase from the venom of Trimeresurus mucrosquamatus (Taiwan habu snake) (1988), Toxicon, 26, 695-706.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.3.2 freezing, irreversible inactivation Protobothrops mucrosquamatus
1.4.3.2 irreversible inactivation by lyophilization Protobothrops mucrosquamatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.2 benzoic acid
-
Protobothrops mucrosquamatus
1.4.3.2 Cd2+ CdCl2 Protobothrops mucrosquamatus
1.4.3.2 HgCl
-
Protobothrops mucrosquamatus
1.4.3.2 iodoacetamide
-
Protobothrops mucrosquamatus
1.4.3.2 KCN
-
Protobothrops mucrosquamatus
1.4.3.2 MnCl2
-
Protobothrops mucrosquamatus
1.4.3.2 p-Aminobenzoic acid
-
Protobothrops mucrosquamatus
1.4.3.2 p-chloromercuribenzoate
-
Protobothrops mucrosquamatus
1.4.3.2 ZnCl2
-
Protobothrops mucrosquamatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 1.17
-
L-leucine
-
Protobothrops mucrosquamatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.2 Mn2+ MnCl2 inhibits Protobothrops mucrosquamatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 70000
-
2 * 70000, SDS-PAGE Protobothrops mucrosquamatus
1.4.3.2 140000
-
gel filtration Protobothrops mucrosquamatus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Protobothrops mucrosquamatus
-
Taiwan habu snake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2
-
Protobothrops mucrosquamatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
Protobothrops mucrosquamatus
-

Storage Stability

EC Number Storage Stability Organism
1.4.3.2 4°C, 40% v/v glycerol, 3 months Protobothrops mucrosquamatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-amino acid + H2O + O2
-
Protobothrops mucrosquamatus 2-oxo acid + NH3 + H2O2
-
?
1.4.3.2 L-arginine + H2O + O2 low reaction rate Protobothrops mucrosquamatus 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
?
1.4.3.2 L-asparagine + H2O + O2 very low activity Protobothrops mucrosquamatus alpha-ketosuccinamic acid + NH3 + H2O2
-
?
1.4.3.2 L-aspartic acid + H2O + O2 low activity Protobothrops mucrosquamatus oxaloacetate + NH3 + H2O2
-
?
1.4.3.2 L-cystine + H2O + O2 very low activity Protobothrops mucrosquamatus ? + NH3 + H2O2
-
?
1.4.3.2 L-glutamic acid + H2O + O2 low activity Protobothrops mucrosquamatus 2-oxoglutarate + NH3 + H2O2
-
?
1.4.3.2 L-glutamine + H2O + O2 very low activity Protobothrops mucrosquamatus 2-oxoglutaramate + NH3 + H2O2
-
?
1.4.3.2 L-histidine + H2O + O2 low reaction rate Protobothrops mucrosquamatus 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
?
1.4.3.2 L-isoleucine + H2O + O2 low reaction rate Protobothrops mucrosquamatus alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-leucine + H2O + O2 high reaction rate Protobothrops mucrosquamatus 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-methionine + H2O + O2 high reaction rate Protobothrops mucrosquamatus 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
1.4.3.2 L-phenylalanine + H2O + O2 high reaction rate Protobothrops mucrosquamatus phenylpyruvate + NH3 + H2O2
-
?
1.4.3.2 L-tryptophan + H2O + O2 low reaction rate Protobothrops mucrosquamatus indole-3-pyruvate + NH3 + H2O2
-
?
1.4.3.2 L-tyrosine + H2O + O2
-
Protobothrops mucrosquamatus p-hydroxyphenylpyruvate + NH3 + H2O2
-
?
1.4.3.2 L-valine + H2O + O2 very low activity Protobothrops mucrosquamatus 2-oxoisovalerate + NH3 + H2O2
-
?
1.4.3.2 additional information not: proline Protobothrops mucrosquamatus ?
-
?
1.4.3.2 additional information not: L-threonine Protobothrops mucrosquamatus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 dimer 2 * 70000, SDS-PAGE Protobothrops mucrosquamatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.2 50
-
10 min, pH 6.8, stable up to Protobothrops mucrosquamatus
1.4.3.2 65
-
10 min, 54% loss of activity Protobothrops mucrosquamatus
1.4.3.2 75
-
10 min, 97% loss of activity Protobothrops mucrosquamatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 7
-
L-Ile, L-Leu, L-Cys, L-Met, L-Phe, L-Tyr Protobothrops mucrosquamatus
1.4.3.2 8
-
L-His Protobothrops mucrosquamatus
1.4.3.2 9
-
L-Trp Protobothrops mucrosquamatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.2 5
-
4°C, 24 h, complete inactivation Protobothrops mucrosquamatus
1.4.3.2 7 8 4°C, 24 h, stable Protobothrops mucrosquamatus
1.4.3.2 10
-
4°C, 24 h, complete inactivation Protobothrops mucrosquamatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FMN 2 mol of FMN per mol of enzyme Protobothrops mucrosquamatus