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Literature summary extracted from

  • Kusakabe, H.; Kodama, K.; Kuninaka, A.; Yoshino, H.; Misono, H.; Soda, K.
    A new antitumor enzyme, L-lysine alpha-oxidase from Trichoderma viride. Purification and enzymological properties (1980), J. Biol. Chem., 255, 976-981.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.14 medicine antitumor activity Trichoderma viride

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.14 HgCl2
-
 Trichoderma viride
1.4.3.14 p-chloromercuribenzoate
-
 Trichoderma viride

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.14 0.04
-
 L-lysine
-
 Trichoderma viride
1.4.3.14 0.044
-
 L-ornithine
-
 Trichoderma viride
1.4.3.14 1.6
-
 O2
-
 Trichoderma viride
1.4.3.14 14
-
 L-phenylalanine
-
 Trichoderma viride

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.14 extracellular
-
 Trichoderma viride
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.14 56000
-
 2 * 56000, SDS-PAGE Trichoderma viride
1.4.3.14 112000 119000 gel filtration, sedimentation equilibrium centrifugation Trichoderma viride

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.14 L-lysine + O2 + H2O Trichoderma viride also other alpha,omega-diaminomonocarboxylates and L-lysine hydroxamate are oxidized 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.14 Trichoderma viride
-
 mold, grown in wheat bran culture
-
1.4.3.14 Trichoderma viride
-
 Y244-2, ATCC 20536
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.14 ammonium sulfate precipitation, ion-exchange, gel-filtration Trichoderma viride

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.14 additional information
-
 1.2 U/ml Trichoderma viride
1.4.3.14 66.15
-
-
 Trichoderma viride

Storage Stability

EC Number Storage Stability Organism
1.4.3.14 -20°C, 0.1 M potassium phosphate buffer, pH 7.4, at least 3 months, with little loss of activity Trichoderma viride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.14 L-arginine + O2 + H2O
-
 Trichoderma viride 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-histidine + O2 + H2O
-
 Trichoderma viride 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + O2 + H2O also other alpha,omega-diaminomonocarboxylates and L-lysine hydroxamate are oxidized Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-ornithine + O2 + H2O
-
 Trichoderma viride 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.14 L-phenylalanine + O2 + H2O
-
 Trichoderma viride phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.14 L-tyrosine + O2 + H2O
-
 Trichoderma viride 3-(4-hydroxyphenyl)pyruvate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.14 dimer 2 * 56000, SDS-PAGE Trichoderma viride

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.14 37
-
 assay at Trichoderma viride
1.4.3.14 50
-
 for L-lysine Trichoderma viride

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.14 30 70 82% relative activity at 30°C, 103% at 50°C and 58°C at 70°C Trichoderma viride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.14 additional information
-
 heat labile Trichoderma viride
1.4.3.14 45
-
 stable for 30 min in the pH range from pH 5.0 or pH 7.0 to pH 10.0 Trichoderma viride
1.4.3.14 55
-
 0.1 M potassium phosphate buffer, pH 7.4, 30 min, 100% of original activity Trichoderma viride
1.4.3.14 60
-
 0.1 M potassium phosphate buffer, pH 7.4, 30 min, 92% of original activity Trichoderma viride
1.4.3.14 65
-
 0.1 M potassium phosphate buffer, pH 7.4, 30 min, 42% of original activity Trichoderma viride
1.4.3.14 70
-
 0.1 M potassium phosphate buffer, pH 7.4, 30 min, 4% of original activity Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.14 7.4 9.2 for L-lysine, reaction rate declines markedly below pH 7.0 Trichoderma viride
1.4.3.14 8
-
 assay at Trichoderma viride

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.14 FAD 2 mol per mol of enzyme, prosthetic group Trichoderma viride