BRENDA - Enzyme Database

D-aspartate oxidase of kidney

Dixon, M.; Kenworthy, P.; Biochim. Biophys. Acta 146, 54-76 (1967)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.1
2-oxoglutaric acid
at 6.67 mM 64% inhibition
Oryctolagus cuniculus
1.4.3.1
adipic acid
at 6.67 mM 15% inhibition
Oryctolagus cuniculus
1.4.3.1
Citric acid
at 6.67 mM 29% inhibition
Oryctolagus cuniculus
1.4.3.1
D-malate
90% inhibition at 0.33 mM
Oryctolagus cuniculus
1.4.3.1
D-Tartaric acid
at 6.67 mM 77% inhibition
Oryctolagus cuniculus
1.4.3.1
Fumaric acid
at 6.67 mM 78% inhibition
Oryctolagus cuniculus
1.4.3.1
Glutaric acid
at 6.67 mM 62% inhibition
Oryctolagus cuniculus
1.4.3.1
L-leucine
6% inhibition at 6.67 mM
Oryctolagus cuniculus
1.4.3.1
L-Malic acid
at 6.67 mM 79% inhibition
Oryctolagus cuniculus
1.4.3.1
L-Tartaric acid
at 6.67 mM 24% inhibition
Oryctolagus cuniculus
1.4.3.1
L-valine
6% inhibition at 6.67 mM
Oryctolagus cuniculus
1.4.3.1
malonate
at 0.66 mM 78% inhibition
Oryctolagus cuniculus
1.4.3.1
oxalic acid
at 6.67 mM 40% inhibition
Oryctolagus cuniculus
1.4.3.1
oxaloacetic acid
at 6.67 mM 90% inhibition
Oryctolagus cuniculus
1.4.3.1
succinic acid
at 6.67 mM 66% inhibition
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.1
0.86
-
D-Aspartate
O2 as acceptor
Oryctolagus cuniculus
1.4.3.1
5.2
-
D-Aspartate
ferricyanide as acceptor
Oryctolagus cuniculus
1.4.3.1
5.7
-
D-glutamate
ferricyanide as acceptor
Oryctolagus cuniculus
1.4.3.1
166
-
D-glutamate
O2 as acceptor
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
Oryctolagus cuniculus
-
oxaloacetate + NH3 + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.1
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.1
-
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.1
kidney
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.1
2.4
-
ferricyanide as electron acceptor
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
-
391727
Oryctolagus cuniculus
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-aspartate + H2O + O2
ferricyanide and 2,6-dichlorophenolindophenol can also act as electron acceptors instead of O2
391727
Oryctolagus cuniculus
oxaloacetate + NH3 + H2O2
-
391727
Oryctolagus cuniculus
?
1.4.3.1
D-glutamate + H2O + O2
ferricyanide and 2,6-dichlorophenolindophenol can also act as electron acceptors instead of O2
391727
Oryctolagus cuniculus
2-oxoglutarate + NH3 + H2O2
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.1
additional information
-
optimum depends on the order of addition of the reactands
Oryctolagus cuniculus
1.4.3.1
9.5
-
-
Oryctolagus cuniculus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.4.3.1
4.5
11
largely destroyed below or over this range
Oryctolagus cuniculus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.1
6
8.5
fairly stable
Oryctolagus cuniculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.1
0.011
-
D-malate
-
Oryctolagus cuniculus
1.4.3.1
0.06
-
malonate
-
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.1
2-oxoglutaric acid
at 6.67 mM 64% inhibition
Oryctolagus cuniculus
1.4.3.1
adipic acid
at 6.67 mM 15% inhibition
Oryctolagus cuniculus
1.4.3.1
Citric acid
at 6.67 mM 29% inhibition
Oryctolagus cuniculus
1.4.3.1
D-malate
90% inhibition at 0.33 mM
Oryctolagus cuniculus
1.4.3.1
D-Tartaric acid
at 6.67 mM 77% inhibition
Oryctolagus cuniculus
1.4.3.1
Fumaric acid
at 6.67 mM 78% inhibition
Oryctolagus cuniculus
1.4.3.1
Glutaric acid
at 6.67 mM 62% inhibition
Oryctolagus cuniculus
1.4.3.1
L-leucine
6% inhibition at 6.67 mM
Oryctolagus cuniculus
1.4.3.1
L-Malic acid
at 6.67 mM 79% inhibition
Oryctolagus cuniculus
1.4.3.1
L-Tartaric acid
at 6.67 mM 24% inhibition
Oryctolagus cuniculus
1.4.3.1
L-valine
6% inhibition at 6.67 mM
Oryctolagus cuniculus
1.4.3.1
malonate
at 0.66 mM 78% inhibition
Oryctolagus cuniculus
1.4.3.1
oxalic acid
at 6.67 mM 40% inhibition
Oryctolagus cuniculus
1.4.3.1
oxaloacetic acid
at 6.67 mM 90% inhibition
Oryctolagus cuniculus
1.4.3.1
succinic acid
at 6.67 mM 66% inhibition
Oryctolagus cuniculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.1
0.011
-
D-malate
-
Oryctolagus cuniculus
1.4.3.1
0.06
-
malonate
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.1
0.86
-
D-Aspartate
O2 as acceptor
Oryctolagus cuniculus
1.4.3.1
5.2
-
D-Aspartate
ferricyanide as acceptor
Oryctolagus cuniculus
1.4.3.1
5.7
-
D-glutamate
ferricyanide as acceptor
Oryctolagus cuniculus
1.4.3.1
166
-
D-glutamate
O2 as acceptor
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
Oryctolagus cuniculus
-
oxaloacetate + NH3 + H2O2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.1
-
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.1
kidney
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.1
2.4
-
ferricyanide as electron acceptor
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
-
391727
Oryctolagus cuniculus
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-aspartate + H2O + O2
ferricyanide and 2,6-dichlorophenolindophenol can also act as electron acceptors instead of O2
391727
Oryctolagus cuniculus
oxaloacetate + NH3 + H2O2
-
391727
Oryctolagus cuniculus
?
1.4.3.1
D-glutamate + H2O + O2
ferricyanide and 2,6-dichlorophenolindophenol can also act as electron acceptors instead of O2
391727
Oryctolagus cuniculus
2-oxoglutarate + NH3 + H2O2
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.1
additional information
-
optimum depends on the order of addition of the reactands
Oryctolagus cuniculus
1.4.3.1
9.5
-
-
Oryctolagus cuniculus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.4.3.1
4.5
11
largely destroyed below or over this range
Oryctolagus cuniculus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.1
6
8.5
fairly stable
Oryctolagus cuniculus