BRENDA - Enzyme Database

Purification and properties of D-aspartate oxidase from Cryptococcus humicolus UJ1

Yamada, R.h.; Ujiie, H.; Kera, Y.; Nakase, T.; Kitagawa, K.; Imasaka, T.; Arimoto, K.; Takahashi, M.; Matsumura, Y.; Biochim. Biophys. Acta 1294, 153-158 (1996)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.1
D-malate
competitive inhibitor, at 20 mM 34% inhibition
Vanrija humicola
1.4.3.1
malonate
competitive inhibitor, at 20 mM 53% inhibition
Vanrija humicola
1.4.3.1
meso-tartrate
9% inhibition at 20 mM
Vanrija humicola
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.1
3.7
-
D-Aspartate
-
Vanrija humicola
1.4.3.1
28
-
N-Methyl-D-aspartate
-
Vanrija humicola
1.4.3.1
152
-
D-glutamate
-
Vanrija humicola
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.1
40000
-
SDS-PAGE
Vanrija humicola
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
Vanrija humicola
-
oxaloacetate + NH3 + H2O2
-
-
?
1.4.3.1
D-aspartate + H2O + O2
Vanrija humicola UJ1
-
oxaloacetate + NH3 + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.1
Vanrija humicola
-
-
-
1.4.3.1
Vanrija humicola UJ1
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.1
-
Vanrija humicola
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.1
76.1
-
-
Vanrija humicola
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
-
391713
Vanrija humicola
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-aspartate + H2O + O2
-
391713
Vanrija humicola
oxaloacetate + NH3 + H2O2
-
391713
Vanrija humicola
?
1.4.3.1
D-aspartate + H2O + O2
-
391713
Vanrija humicola UJ1
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-aspartate + H2O + O2
-
391713
Vanrija humicola UJ1
oxaloacetate + NH3 + H2O2
-
391713
Vanrija humicola UJ1
?
1.4.3.1
D-glutamate + H2O + O2
-
391713
Vanrija humicola
2-oxoglutarate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-glutamate + H2O + O2
-
391713
Vanrija humicola UJ1
2-oxoglutarate + NH3 + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-aspartate + H2O + O2
-
391713
Vanrija humicola
oxaloacetate + CH3NH2 + H2O2
-
391713
Vanrija humicola
?
1.4.3.1
N-methyl-D-aspartate + H2O + O2
-
391713
Vanrija humicola UJ1
oxaloacetate + CH3NH2 + H2O2
-
391713
Vanrija humicola UJ1
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.3.1
homotetramer
gel filtration
Vanrija humicola
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.3.1
33
37
-
Vanrija humicola
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.1
7.5
-
-
Vanrija humicola
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.1
FAD
-
Vanrija humicola
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.1
5.4
-
malonate
-
Vanrija humicola
1.4.3.1
13.3
-
D-malate
-
Vanrija humicola
1.4.3.1
61
-
meso-tartrate
-
Vanrija humicola
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.1
FAD
-
Vanrija humicola
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.1
D-malate
competitive inhibitor, at 20 mM 34% inhibition
Vanrija humicola
1.4.3.1
malonate
competitive inhibitor, at 20 mM 53% inhibition
Vanrija humicola
1.4.3.1
meso-tartrate
9% inhibition at 20 mM
Vanrija humicola
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.1
5.4
-
malonate
-
Vanrija humicola
1.4.3.1
13.3
-
D-malate
-
Vanrija humicola
1.4.3.1
61
-
meso-tartrate
-
Vanrija humicola
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.1
3.7
-
D-Aspartate
-
Vanrija humicola
1.4.3.1
28
-
N-Methyl-D-aspartate
-
Vanrija humicola
1.4.3.1
152
-
D-glutamate
-
Vanrija humicola
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.1
40000
-
SDS-PAGE
Vanrija humicola
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
Vanrija humicola
-
oxaloacetate + NH3 + H2O2
-
-
?
1.4.3.1
D-aspartate + H2O + O2
Vanrija humicola UJ1
-
oxaloacetate + NH3 + H2O2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.1
-
Vanrija humicola
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.1
76.1
-
-
Vanrija humicola
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
-
391713
Vanrija humicola
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-aspartate + H2O + O2
-
391713
Vanrija humicola
oxaloacetate + NH3 + H2O2
-
391713
Vanrija humicola
?
1.4.3.1
D-aspartate + H2O + O2
-
391713
Vanrija humicola UJ1
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-aspartate + H2O + O2
-
391713
Vanrija humicola UJ1
oxaloacetate + NH3 + H2O2
-
391713
Vanrija humicola UJ1
?
1.4.3.1
D-glutamate + H2O + O2
-
391713
Vanrija humicola
2-oxoglutarate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-glutamate + H2O + O2
-
391713
Vanrija humicola UJ1
2-oxoglutarate + NH3 + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-aspartate + H2O + O2
-
391713
Vanrija humicola
oxaloacetate + CH3NH2 + H2O2
-
391713
Vanrija humicola
?
1.4.3.1
N-methyl-D-aspartate + H2O + O2
-
391713
Vanrija humicola UJ1
oxaloacetate + CH3NH2 + H2O2
-
391713
Vanrija humicola UJ1
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.3.1
homotetramer
gel filtration
Vanrija humicola
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.3.1
33
37
-
Vanrija humicola
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.1
7.5
-
-
Vanrija humicola