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Literature summary extracted from

  • Negri, A.; Tedeschi, G.; Ceciliani, F.; Ronchi, S.
    Purification of beef kidney D-aspartate oxidase overexpressed in Escherichia coli and characterization of its redox potentials and oxidative activity towards agonists and antagonists of excitatory amino acid receptors (1999), Biochim. Biophys. Acta, 1431, 212-222.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.1 expressed in Escherichia coli GI724 cells Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.1 L-Tartrate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.1 1.5
-
 N-Methyl-D-aspartate
-
 Bos taurus
1.4.3.1 1.7
-
 DL-cysteic acid
-
 Bos taurus
1.4.3.1 3
-
 D-aspartic acid-beta-hydroxamate
-
 Bos taurus
1.4.3.1 3.7
-
 D-Aspartate
-
 Bos taurus
1.4.3.1 4.5
-
 cis-2,3-piperidine dicarboxylic acid
-
 Bos taurus
1.4.3.1 5.6
-
 D-glutamate
-
 Bos taurus
1.4.3.1 9.8
-
 D-homocysteic acid
-
 Bos taurus
1.4.3.1 15.2
-
 DL-2-amino-3-phosphonopropanoic acid
-
 Bos taurus
1.4.3.1 16.2
-
 D-alpha-aminoadipic acid
-
 Bos taurus
1.4.3.1 143
-
 glycyl-D-aspartic acid
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.1 37659
-
 1 * 37659, amino acid analysis Bos taurus
1.4.3.1 37660
-
 apoprotein, amino acid analysis Bos taurus
1.4.3.1 38000
-
 recombinant protein, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.1 D-aspartate + H2O + O2 Bos taurus
-
 oxaloacetate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.1 expressed in Escherichia coli, mixture of 80-91% active FAD-containing and 9-20% inactive OH-FAD containing enzyme Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.1 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.1 29.1
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.1 cis-2,3-piperidine dicarboxylic acid + H2O + O2
-
 Bos taurus ?
-
 ?
1.4.3.1 D-alpha-aminoadipic acid + H2O + O2
-
 Bos taurus 2-oxoadipic acid + NH3 + H2O2
-
 ?
1.4.3.1 D-aspartate + H2O + O2
-
 Bos taurus oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.1 D-aspartic acid-beta-hydroxamate + H2O + O2
-
 Bos taurus 4-(hydroxyamino)-2,4-dioxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.1 D-glutamate + H2O + O2
-
 Bos taurus 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.1 D-homocysteic acid + H2O + O2
-
 Bos taurus 2-oxo-4-sulfobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.1 DL-2-amino-3-phosphonopropanoic acid + H2O + O2
-
 Bos taurus 2-oxo-3-phosphonopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.1 DL-cysteic acid + H2O + O2
-
 Bos taurus 2-oxo-3-sulfopropionic acid + NH3 + H2O2
-
 ?
1.4.3.1 glycyl-D-aspartic acid + H2O + O2
-
 Bos taurus ?
-
 ?
1.4.3.1 N-methyl-D-aspartate + H2O + O2
-
 Bos taurus oxaloacetate + CH3NH2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.1 monomer 1 * 37659, amino acid analysis Bos taurus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.1 DASPO
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.1 FAD
-
 Bos taurus