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Literature summary extracted from

  • Sanders, H.K.; Becker, G.E.; Nason, A.
    Glycine-cytochrome c reductase from Nitrobacter agilis (1972), J. Biol. Chem., 247, 2015-2025.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.2.1 Cu2+ 55% inhibition at 1 mM Nitrobacter winogradskyi
1.4.2.1 cytochrome c above 0.15 mM Nitrobacter winogradskyi
1.4.2.1 diphosphate buffer, 10 mM Nitrobacter winogradskyi
1.4.2.1 EDTA 64% inhibition at 7.1 mM Nitrobacter winogradskyi
1.4.2.1 hydrazine
-
 Nitrobacter winogradskyi
1.4.2.1 hydroxylamine
-
 Nitrobacter winogradskyi
1.4.2.1 KCl 80% inhibition at 75 mM Nitrobacter winogradskyi
1.4.2.1 KCN 47% inhibition at 7.1 mM Nitrobacter winogradskyi
1.4.2.1 KNO3 80% inhibition at 75 mM Nitrobacter winogradskyi
1.4.2.1 MgCl2 80% inhibition at 40 mM Nitrobacter winogradskyi
1.4.2.1 NaCl 80% inhibition at 75 mM Nitrobacter winogradskyi
1.4.2.1 phosphate buffer, 100 mM Nitrobacter winogradskyi
1.4.2.1 pyridoxal 5'-phosphate up to 80% inhibition at 0.2-2 mM due to reaction with glycine Nitrobacter winogradskyi
1.4.2.1 pyruvate 70% inhibition at 10 mM and 20% inhibition at 3 mM Nitrobacter winogradskyi
1.4.2.1 Quinacrine hydrochloride 0.3-1.0 mM Nitrobacter winogradskyi
1.4.2.1 Sodium azide 10% inhibition at 2 mM Nitrobacter winogradskyi
1.4.2.1 sodium barbital buffer, 100 mM, pH 8.0 Nitrobacter winogradskyi
1.4.2.1 Tris buffer, 100 mM, pH 8.0 Nitrobacter winogradskyi
1.4.2.1 Urea 30% inhibition at 50 mM Nitrobacter winogradskyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.2.1 0.016
-
 ferricytochrome c
-
 Nitrobacter winogradskyi
1.4.2.1 0.8
-
 glycine
-
 Nitrobacter winogradskyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.2.1 69000
-
 gel filtration, sucrose density gradient centrifugation Nitrobacter winogradskyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.2.1 glycine + ferricytochrome c Nitrobacter winogradskyi
-
 glyoxylate + ferrocytochrome c + NH3
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.4.2.1 Nitrobacter winogradskyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.2.1
-
 Nitrobacter winogradskyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.2.1 13.1
-
-
 Nitrobacter winogradskyi

Storage Stability

EC Number Storage Stability Organism
1.4.2.1 4°C, 4-10 weeks, 30-50% loss of activity Nitrobacter winogradskyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.2.1 glycine + ferricytochrome c
-
 Nitrobacter winogradskyi glyoxylate + ferrocytochrome c + NH3
-
 ir
1.4.2.1 glycine + ferricytochrome c highly specific Nitrobacter winogradskyi glyoxylate + ferrocytochrome c + NH3
-
 ir
1.4.2.1 glycine + H2O + ferricytochrome c
-
 Nitrobacter winogradskyi glyoxylate + NH3 + ferrocytochrome c + H+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.2.1 4
-
 4-10 weeks, 30-50% loss of activity Nitrobacter winogradskyi
1.4.2.1 70
-
 5 min: 75% loss of activity, 2 min: 50% loss of activity Nitrobacter winogradskyi
1.4.2.1 80
-
 2 min: 100% loss of activity Nitrobacter winogradskyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.2.1 6.1 6.9 in potassium phosphate or diphosphate buffer Nitrobacter winogradskyi