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Literature summary extracted from

  • Sakamoto, N.; Kotre, A.M.; Savageau, M.A.
    Glutamate dehydrogenase from Escherichia coli: purification and properties (1975), J. Bacteriol., 124, 775-783.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.4.1.4 inactivated by freezing/thawing Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 guanidine hydrochloride complete loss of activity Escherichia coli
1.4.1.4 L-homoserine competitive inhibitor with respect to both ammonia and glutamine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.04
-
 NADPH reductive amination Escherichia coli
1.4.1.4 0.042
-
 NADP+ oxidative deamination Escherichia coli
1.4.1.4 0.64
-
 2-oxoglutarate reductive amination Escherichia coli
1.4.1.4 1.1
-
 NH4+ reductive amination Escherichia coli
1.4.1.4 1.3
-
 L-glutamate oxidative deamination Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 50000
-
 alpha6, 6 * 50000, SDS-PAGE Escherichia coli
1.4.1.4 300000
-
 sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Escherichia coli
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 180
-
 oxidative deamination at 25°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.4.1.4 4°C, water, 20 mM potassium phosphate or sodium phosphate, pH 7.2, at least 3 months Escherichia coli
1.4.1.4 room temperature, several months stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Escherichia coli L-glutamate + NADP+
-
 ?
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Escherichia coli 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homohexamer alpha6, 6 * 50000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4
-
-
 inactivated by freezing Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Escherichia coli
1.4.1.4 NADPH
-
 Escherichia coli