Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Veronese, F.M.; Boccu, E.; Conventi, L.
    Glutamate dehydrogenase from Escherichia coli: induction, purification and properties of the enzyme (1975), Biochim. Biophys. Acta, 377, 217-228.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.4 AMP slight activation up to 1 mM Escherichia coli
1.4.1.4 ATP at 2 mM, 50% activation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 AMP inhibitory at higher concentrations than 1 mM Escherichia coli
1.4.1.4 Urea at 8 M stable for 10 min Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 46000
-
 x * 46000, SDS-PAGE Escherichia coli
1.4.1.4 245000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Escherichia coli
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Escherichia coli MRE 600
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Escherichia coli
-
-
-
1.4.1.4 Escherichia coli MRE 600
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 250
-
 oxidative deamination Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.4.1.4 4°C, 0.1 M phosphate buffer, several days, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Escherichia coli 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Escherichia coli MRE 600 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 ? x * 46000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8
-
 reductive amination Escherichia coli
1.4.1.4 9
-
 oxidative deamination Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Escherichia coli
1.4.1.4 NADPH
-
 Escherichia coli