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Literature summary extracted from
- Purification and some properties of the NADP-linked glutamate dehydrogenase from Trypanosoma cruzi (1978), Int. J. Biochem., 9, 395-400.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | KCl | up to 50mM | Trypanosoma cruzi |  |
| 1.4.1.4 | NaCl | up to 50 mM | Trypanosoma cruzi | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | KCl | more than 50 mM | Trypanosoma cruzi | |
| 1.4.1.4 | NaCl | more than 50 mM | Trypanosoma cruzi | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.4 | 0.004 | - |
NADPH | reductive amination | Trypanosoma cruzi | |
| 1.4.1.4 | 0.0064 | - |
NADP+ | oxidative deamination | Trypanosoma cruzi | |
| 1.4.1.4 | 1 | - |
2-oxoglutarate | reductive amination | Trypanosoma cruzi | |
| 1.4.1.4 | 1.7 | - |
NH4+ | reductive amination | Trypanosoma cruzi | |
| 1.4.1.4 | 2 | - |
L-glutamate | reductive amination | Trypanosoma cruzi | |
| 1.4.1.4 | 66 | - |
NH4+ | reductive amination | Trypanosoma cruzi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 64000 | - |
4 * 64000, SDS-PAGE | Trypanosoma cruzi |
| 1.4.1.4 | 280000 | - |
gel filtration | Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.4 | L-glutamate + NADP+ + H2O | Trypanosoma cruzi | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.4 | Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.4 | - |
Trypanosoma cruzi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 51.2 | - |
reductive amination | Trypanosoma cruzi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.4 | 2-oxoglutarate + NH4+ + NADPH | - |
Trypanosoma cruzi | L-glutamate + NADP+ | - |
? | |
| 1.4.1.4 | L-glutamate + NADP+ + H2O | - |
Trypanosoma cruzi | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| 1.4.1.4 | L-glutamate + NADP+ + H2O | - |
Trypanosoma cruzi | 2-oxoglutarate + NADPH + NH3 | biphasic kinetic behavior for ammonia | r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.1.4 | homotetramer | 4 * 64000, SDS-PAGE | Trypanosoma cruzi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 7 | - |
oxidative deamination | Trypanosoma cruzi |
| 1.4.1.4 | 8 | - |
reductive amination | Trypanosoma cruzi |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | NADP+ | - |
Trypanosoma cruzi | |
| 1.4.1.4 | NADPH | - |
Trypanosoma cruzi | |
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