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Literature summary extracted from

  • Misono, H.; Goto, N.; Nagasaki, S.
    Purification, crystallization and properties of NADP+-specific glutamate dehydrogenase from Lactobacillus fermentum (1985), Agric. Biol. Chem., 49, 117-123.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.4
-
 Limosilactobacillus fermentum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.044
-
 NADP+ oxidative deamination Limosilactobacillus fermentum
1.4.1.4 0.078
-
 NADPH reductive amination Limosilactobacillus fermentum
1.4.1.4 5.6
-
 2-oxoglutarate reductive amination Limosilactobacillus fermentum
1.4.1.4 6.76
-
 NH4+ reductive amination Limosilactobacillus fermentum
1.4.1.4 79
-
 L-glutamate oxidative deamination Limosilactobacillus fermentum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 50000
-
 alpha6, 6 * 50000, SDS-PAGE Limosilactobacillus fermentum
1.4.1.4 300000
-
 gel filtration Limosilactobacillus fermentum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Lactococcus lactis
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Streptococcus equinus
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Enterococcus faecalis
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Limosilactobacillus fermentum
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Streptococcus equinus IFO 12553
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Lactococcus lactis IFO 12546
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Enterococcus faecalis IFO 12964
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Limosilactobacillus fermentum IFO 3071
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Enterococcus faecalis
-
-
-
1.4.1.4 Enterococcus faecalis IFO 12964
-
-
-
1.4.1.4 Lactococcus lactis
-
-
-
1.4.1.4 Lactococcus lactis IFO 12546
-
-
-
1.4.1.4 Limosilactobacillus fermentum
-
-
-
1.4.1.4 Limosilactobacillus fermentum IFO 3071
-
-
-
1.4.1.4 Streptococcus equinus
-
-
-
1.4.1.4 Streptococcus equinus IFO 12553
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Limosilactobacillus fermentum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 57.6
-
 reductive amination Limosilactobacillus fermentum

Storage Stability

EC Number Storage Stability Organism
1.4.1.4 -20°C, potassium phosphate buffer, pH 7.4, containing 0.01% 2-mercaptoethanol and 10% glycerol, 100% activity Limosilactobacillus fermentum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Limosilactobacillus fermentum L-glutamate + NADP+
-
 ?
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Limosilactobacillus fermentum IFO 3071 L-glutamate + NADP+
-
 ?
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Lactococcus lactis 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Streptococcus equinus 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Enterococcus faecalis 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Limosilactobacillus fermentum 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Streptococcus equinus IFO 12553 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Lactococcus lactis IFO 12546 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Enterococcus faecalis IFO 12964 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Limosilactobacillus fermentum IFO 3071 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homohexamer alpha6, 6 * 50000, SDS-PAGE Limosilactobacillus fermentum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 70
-
 after 10 min in phosphate buffer pH 7.4 100% activity Limosilactobacillus fermentum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8
-
 reductive amination Limosilactobacillus fermentum
1.4.1.4 9
-
 oxidative deamination Limosilactobacillus fermentum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Lactococcus lactis
1.4.1.4 NADP+
-
 Streptococcus equinus
1.4.1.4 NADP+
-
 Enterococcus faecalis
1.4.1.4 NADP+
-
 Limosilactobacillus fermentum
1.4.1.4 NADPH
-
 Lactococcus lactis
1.4.1.4 NADPH
-
 Streptococcus equinus
1.4.1.4 NADPH
-
 Enterococcus faecalis
1.4.1.4 NADPH
-
 Limosilactobacillus fermentum