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Literature summary extracted from

  • Martinez-Bilbao, M.; Martinez, A.; Urkijo, I.; Llama, M.J.; Serra, J.L.
    Induction, isolation, and some properties of the NADPH-dependent glutamate dehydrogenase from the nonheterocystous cyanobacterium Phormidium laminosum (1988), J. Bacteriol., 170, 4897-4902.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.4.1.4 unstable when frozen at -20°C after a few h even with addition of 50% v/v glycerol Phormidium laminosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 Glutaric acid at 20 mM, 25% inhibition Phormidium laminosum
1.4.1.4 L-Glutamic acid at 20 mM 25% inhibition Phormidium laminosum
1.4.1.4 L-tryptophan at 20 mM, 15% inhibition Phormidium laminosum
1.4.1.4 Urea no activity at 6 mM Phormidium laminosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.064
-
 NADPH reductive amination Phormidium laminosum
1.4.1.4 1.25
-
 2-oxoglutarate reductive amination Phormidium laminosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 280000
-
 gel filtration Phormidium laminosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Phormidium laminosum
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Phormidium laminosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Phormidium laminosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 32.9
-
 reductive amination at 30°C Phormidium laminosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Phormidium laminosum 2-oxoglutarate + NADPH + NH3
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 60
-
 reductive amination Phormidium laminosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 72
-
 complete inactivation Phormidium laminosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 7.5
-
 reductive amination in potassium phosphate buffer Phormidium laminosum
1.4.1.4 8
-
 reductive amination in Tris hydrochloride buffer Phormidium laminosum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Phormidium laminosum
1.4.1.4 NADPH
-
 Phormidium laminosum