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Literature summary extracted from

  • Rahman, R.N.Z.A.; Fujiwara, S.; Nakamura, H.; Takagi, M.; Imanaka, T.
    Ion pairs involved in maintaining a thermostable structure of glutamate dehydrogenase from a hyperthermophilic archaeon (1998), Biochem. Biophys. Res. Commun., 248, 920-926.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.4 expression in Escherichia coli BL21 Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.4 E158Q 3.3% as active as wild-type Thermococcus kodakarensis
1.4.1.4 T138E 1.6% as active as wild-type Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 47300
-
alpha6, 6 * 47300, SDS-PAGE Thermococcus kodakarensis
1.4.1.4 284000
-
gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Thermococcus kodakarensis
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Thermococcus kodakarensis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 40
-
T138E-mutant Thermococcus kodakarensis
1.4.1.4 80
-
E158Q-mutant Thermococcus kodakarensis
1.4.1.4 2500
-
wild-type Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O
-
Thermococcus kodakarensis 2-oxoglutarate + NADPH + NH3
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homohexamer alpha6, 6 * 47300, SDS-PAGE Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 60
-
E158Q-mutant Thermococcus kodakarensis
1.4.1.4 85
-
T138E-mutant Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 100
-
wild-type and T138E 50% activity after 2 h and 3 h respectively Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
Thermococcus kodakarensis
1.4.1.4 NADPH
-
Thermococcus kodakarensis