EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.4 | expression in Escherichia coli BL21 | Thermococcus kodakarensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.1.4 | E158Q | 3.3% as active as wild-type | Thermococcus kodakarensis |
1.4.1.4 | T138E | 1.6% as active as wild-type | Thermococcus kodakarensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 47300 | - |
alpha6, 6 * 47300, SDS-PAGE | Thermococcus kodakarensis |
1.4.1.4 | 284000 | - |
gel filtration | Thermococcus kodakarensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | L-glutamate + NADP+ + H2O | Thermococcus kodakarensis | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.4 | Thermococcus kodakarensis | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 40 | - |
T138E-mutant | Thermococcus kodakarensis |
1.4.1.4 | 80 | - |
E158Q-mutant | Thermococcus kodakarensis |
1.4.1.4 | 2500 | - |
wild-type | Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | L-glutamate + NADP+ + H2O | - |
Thermococcus kodakarensis | 2-oxoglutarate + NADPH + NH3 | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.4 | homohexamer | alpha6, 6 * 47300, SDS-PAGE | Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 60 | - |
E158Q-mutant | Thermococcus kodakarensis |
1.4.1.4 | 85 | - |
T138E-mutant | Thermococcus kodakarensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 100 | - |
wild-type and T138E 50% activity after 2 h and 3 h respectively | Thermococcus kodakarensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | NADP+ | - |
Thermococcus kodakarensis | |
1.4.1.4 | NADPH | - |
Thermococcus kodakarensis |