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Literature summary extracted from

  • Thatcher, B.J.; Storey, K.B.
    Glutamate dehydrogenase from liver of euthermic and hibernating Richardson's ground squirrels: Evidence for two distinct enzyme forms (2001), Biochem. Cell Biol., 79, 11-19.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.3 ADP
-
 Bos taurus
1.4.1.3 ADP
-
 Urocitellus richardsonii
1.4.1.3 AMP
-
 Bos taurus
1.4.1.3 AMP
-
 Urocitellus richardsonii
1.4.1.3 KCl activates at 5°C and 37°C Urocitellus richardsonii
1.4.1.3 leucine activates at 5°C only Urocitellus richardsonii
1.4.1.3 NaCl activates at 37°C only Urocitellus richardsonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.3 ATP strong inhibition at 37°C, no inhibition at 5°C Bos taurus
1.4.1.3 ATP strong inhibition at 37°C, no inhibition at 5°C Urocitellus richardsonii
1.4.1.3 GTP
-
 Bos taurus
1.4.1.3 GTP strong inhibition at 37°C, weak inhibition at 5°C Urocitellus richardsonii
1.4.1.3 additional information complex inhibition pattern for ATP, GTP, NaCl, KCl, sodium acetate, NaI, and potassium nitrate of forward and reverse reaction at 5°C and 37°C Bos taurus
1.4.1.3 additional information complex inhibition pattern for ATP, GTP, NaCl, KCl, sodium acetate, NaI, and potassium nitrate of forward and reverse reaction at 5°C and 37°C Urocitellus richardsonii
1.4.1.3 NaCl
-
 Bos taurus
1.4.1.3 Sodium acetate at 5°C only Urocitellus richardsonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.3 0.1
-
 2-oxoglutarate euthermic animal, assay at 37°C Urocitellus richardsonii
1.4.1.3 0.25
-
 glutamate hibernating animal, assay at 5°C Urocitellus richardsonii
1.4.1.3 0.43
-
 2-oxoglutarate hibernating animal, assay at 5°C Urocitellus richardsonii
1.4.1.3 0.47
-
 glutamate assay at 5°C Urocitellus richardsonii
1.4.1.3 0.5
-
 glutamate euthermic animal, assay at 5°C Urocitellus richardsonii
1.4.1.3 0.98
-
 2-oxoglutarate hibernating animal, assay at 37°C Urocitellus richardsonii
1.4.1.3 1.4
-
 2-oxoglutarate
-
 Bos taurus
1.4.1.3 1.4
-
 2-oxoglutarate assay at 37°C Urocitellus richardsonii
1.4.1.3 2.03
-
 glutamate euthermic animal, assay at 37°C Urocitellus richardsonii
1.4.1.3 2.6
-
 glutamate assay at 37°C Urocitellus richardsonii
1.4.1.3 3.66
-
 2-oxoglutarate euthermic animal, assay at 5°C Urocitellus richardsonii
1.4.1.3 4.1
-
 NH4+
-
 Bos taurus
1.4.1.3 4.1
-
 NH4+ assay at 5°C Urocitellus richardsonii
1.4.1.3 5.2
-
 glutamate hibernating animal, assay at 37°C Urocitellus richardsonii
1.4.1.3 7
-
 NH4+ euthermic animal, assay at 37°C Urocitellus richardsonii
1.4.1.3 10.5
-
 2-oxoglutarate
-
 Bos taurus
1.4.1.3 10.5
-
 2-oxoglutarate assay at 5°C Urocitellus richardsonii
1.4.1.3 12.1
-
 NH4+ hibernating animal, assay at 5°C Urocitellus richardsonii
1.4.1.3 15.8
-
 NH4+ hibernating animal, assay at 37°C Urocitellus richardsonii
1.4.1.3 17.8
-
 NH4+
-
 Bos taurus
1.4.1.3 17.8
-
 NH4+ assay at 37°C Urocitellus richardsonii
1.4.1.3 24.7
-
 NH4+ euthermic animal, assay at 5°C Urocitellus richardsonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 59500
-
 6 * 59500, enzyme from both euthermic and hibernating animals Urocitellus richardsonii
1.4.1.3 320000
-
 enzyme from hibernating animals, gel filtration Urocitellus richardsonii
1.4.1.3 331000
-
 gel filtration Bos taurus
1.4.1.3 335000
-
 enzyme from euthermic animals, gel filtration Urocitellus richardsonii

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Bos taurus
-
-
-
1.4.1.3 Urocitellus richardsonii
-
 Richardson's ground squirrel
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3 enzyme from euthermic and hibernating animals Urocitellus richardsonii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.3 liver
-
 Bos taurus
-
1.4.1.3 liver from euthermic, 37°C body temperature, and hibernating, 5°C body temperature, animals Urocitellus richardsonii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 190
-
 enzyme from euthermic animals Urocitellus richardsonii
1.4.1.3 201
-
 enzyme from hibernating animals Urocitellus richardsonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Bos taurus 2-oxoglutarate + NH3 + NAD(P)H
-
 r
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Urocitellus richardsonii 2-oxoglutarate + NH3 + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer 6 * 59500, enzyme from both euthermic and hibernating animals Urocitellus richardsonii