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Literature summary extracted from

  • Diruggiero, J.; Robb, F.T.
    Expression and in vitro assembly of recombinant glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (1995), Appl. Environ. Microbiol., 61, 159-164.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.3 expression in Escherichia coli Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 46000
-
 6 * 46000, recombinant enzyme, SDS-PAGE Pyrococcus furiosus
1.4.1.3 270000
-
 recombinant enzyme, only hexameric form is enzymatically active, gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3 native and recombinant enzyme Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 411
-
 native enzyme at 100°C Pyrococcus furiosus
1.4.1.3 419
-
 recombinant enzyme at 75°C Pyrococcus furiosus
1.4.1.3 423
-
 recombinant enzyme at 90°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Pyrococcus furiosus 2-oxoglutarate + NH3 + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer 6 * 46000, recombinant enzyme, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.3 85
-
 native and recombinant enzyme Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.3 30 90
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.3 100
-
 native enzyme, half-life at 100°C: 10.5 h, recombinant enzyme, half-life at 75°C: 7h, at 90°C: 8.1 h Pyrococcus furiosus