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Literature summary extracted from

  • Kujo, C.; Ohshima, T.
    Enzymological characteristics of the hyperthermostable NAD-dependent glutamate dehydrogenase from the archaeon Pyrobaculum islandicum and effects of denaturants and organic solvents (1998), Appl. Environ. Microbiol., 64, 2152-2157.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.2 acetonitrile activates Pyrobaculum islandicum
1.4.1.2 guanidine hydrochloride activates Pyrobaculum islandicum
1.4.1.2 tetrahydrofuran activates Pyrobaculum islandicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.2 0.005
-
 NADH
-
 Pyrobaculum islandicum
1.4.1.2 0.025
-
 NAD+
-
 Pyrobaculum islandicum
1.4.1.2 0.066
-
 2-oxoglutarate
-
 Pyrobaculum islandicum
1.4.1.2 0.17
-
 L-glutamate
-
 Pyrobaculum islandicum
1.4.1.2 9.7
-
 NH4+
-
 Pyrobaculum islandicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 36000
-
 6 * 36000, SDS-PAGE Pyrobaculum islandicum
1.4.1.2 220000
-
 gel filtration Pyrobaculum islandicum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Pyrobaculum islandicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2
-
 Pyrobaculum islandicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 2-oxoglutarate + NH4+ + NADPH
-
 Pyrobaculum islandicum L-glutamate + NADP+
-
 ?
1.4.1.2 L-glutamate + H2O + NAD+
-
 Pyrobaculum islandicum 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.2 hexamer 6 * 36000, SDS-PAGE Pyrobaculum islandicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.2 90
-
-
 Pyrobaculum islandicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.2 100
-
 no loss of activity after 2 h Pyrobaculum islandicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+
-
 Pyrobaculum islandicum