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Literature summary extracted from

  • Hammer, B.A.; Johnson, E.A.
    Purification, properties, and metabolic roles of NAD+-glutamate dehydrogenase in Clostridium botulinum 113B (1988), Arch. Microbiol., 150, 460-464.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 5,5'-dithiobis(2-nitrobenzoate)
-
 Clostridium botulinum
1.4.1.2 ADP
-
 Clostridium botulinum
1.4.1.2 AMP
-
 Clostridium botulinum
1.4.1.2 ATP
-
 Clostridium botulinum
1.4.1.2 fumarate amination Clostridium botulinum
1.4.1.2 glutamate amination Clostridium botulinum
1.4.1.2 glutamine amination Clostridium botulinum
1.4.1.2 H2O2
-
 Clostridium botulinum
1.4.1.2 Hg2+
-
 Clostridium botulinum
1.4.1.2 oxaloacetate amination Clostridium botulinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 42500
-
 6 * 42500, SDS-PAGE Clostridium botulinum
1.4.1.2 250800
-
 gel filtration Clostridium botulinum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Clostridium botulinum
-
 113B
-
1.4.1.2 Clostridium botulinum 113B
-
 113B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2
-
 Clostridium botulinum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.2 1092
-
 above Clostridium botulinum

Storage Stability

EC Number Storage Stability Organism
1.4.1.2 -20°C, loss of activity overnight Clostridium botulinum
1.4.1.2 4°C, 3 days, stable Clostridium botulinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Clostridium botulinum 2-oxoglutarate + NH3 + NADH + H+
-
 ?
1.4.1.2 L-glutamate + H2O + NAD+
-
 Clostridium botulinum 113B 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.2 hexamer 6 * 42500, SDS-PAGE Clostridium botulinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.2 70
-
 10 min, 40% loss of activity Clostridium botulinum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+
-
 Clostridium botulinum
1.4.1.2 NADH
-
 Clostridium botulinum