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Literature summary extracted from

  • Stone, S.R.; Copeland, L.; Kennedy, I.R.
    Glutamate dehydroenase of lupin nodules: Purification and properties (1979), Phytochemistry, 18, 1273-1278.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 ADP
-
 Lupinus luteus
1.4.1.2 AMP
-
 Lupinus luteus
1.4.1.2 citrate inhibition of amination, no effect on deamination Lupinus luteus
1.4.1.2 EDTA
-
 Lupinus luteus
1.4.1.2 KCN
-
 Lupinus luteus
1.4.1.2 pyridoxal 5'-phosphate
-
 Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 45000
-
 6 * 45000, SDS-PAGE Lupinus luteus
1.4.1.2 270000
-
 gel filtration Lupinus luteus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Lupinus luteus
-
 lupin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2 4 isoenzymes Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.2 nodule
-
 Lupinus luteus
-

Storage Stability

EC Number Storage Stability Organism
1.4.1.2 -20°C, up to 1 year, stable Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Lupinus luteus 2-oxoglutarate + NH3 + NADH + H+
-
 ?
1.4.1.2 L-norvaline + H2O + NAD+ deamination at 5% the rate of L-glutamate deamination Lupinus luteus 2-oxopentanoate + NH3 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.2 hexamer 6 * 45000, SDS-PAGE Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.2 8.2
-
 reductive amination Lupinus luteus
1.4.1.2 8.8
-
 oxidative deamination Lupinus luteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.2 7.9 8.5 about 90% of activity maximum at pH 7.9 and 8.5, reductive amination Lupinus luteus
1.4.1.2 8.4 9.2 about 90% of activity maximum at pH 8.4 and 9.2, oxidative deamination Lupinus luteus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+
-
 Lupinus luteus
1.4.1.2 NADH
-
 Lupinus luteus
1.4.1.2 NADPH can replace NADH only at pH 6, not at pH 7-10 Lupinus luteus