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Literature summary extracted from

  • Misono, H.; Hashimoto, H.; Uehigashi, H.; Nagata, S.; Nagasaki, S.
    Properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens (1989), J. Biochem., 105, 1002-1008.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.18 L-lysine increased activity after 10 min preincubation Agrobacterium tumefaciens

General Stability

EC Number General Stability Organism
1.4.1.18 L-lysine stabilizes Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.18 DTNB almost complete inhibition in presence of NAD+, 40% inhibition in presence of L-lysine Agrobacterium tumefaciens
1.4.1.18 HgCl2 complete inhibition at 0.001 mM after 10 min incubation Agrobacterium tumefaciens
1.4.1.18 N-ethylmaleimide complete inhibition at 1 mM after 10 min incubation Agrobacterium tumefaciens
1.4.1.18 p-chloromercuribenzoate complete inhibition at 0.01 mM after 10 min incubation, reversible with 2-mercaptoethanol or dithiothreitol Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.18 0.059
-
 NAD+
-
 Agrobacterium tumefaciens
1.4.1.18 1.85
-
 deamino-NAD+
-
 Agrobacterium tumefaciens
1.4.1.18 3.13
-
 3-acetylpyridine-NAD+
-
 Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.18 160000
-
 ultracentrifugation in presence of L-lysine Agrobacterium tumefaciens
1.4.1.18 165000
-
 gel filtration in presence of L-lysine Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.18 L-lysine + NAD+ + H2O Agrobacterium tumefaciens
-
 alpha-aminoadipate delta-semialdehyde + NH3 + NADH product is spontaneosly converted into delta1-piperideine-6-carboxylate ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.18 Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.18
-
 Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.18 7.5
-
 preincubation in absence of L-lysine Agrobacterium tumefaciens
1.4.1.18 15.7
-
 preincubation in presence of L-lysine Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.18 L-lysine + 3-acetylpyridine-NAD+ + H2O
-
 Agrobacterium tumefaciens alpha-aminoadipate delta-semialdehyde + NH3 + 3-acetylpyridine-NADH
-
 ?
1.4.1.18 L-lysine + deamino-NAD+ + H2O
-
 Agrobacterium tumefaciens alpha-aminoadipate delta-semialdehyde + NH3 + deamino-NADH
-
 ?
1.4.1.18 L-lysine + NAD+ + H2O
-
 Agrobacterium tumefaciens alpha-aminoadipate delta-semialdehyde + NH3 + NADH product is spontaneosly converted into delta1-piperideine-6-carboxylate ?
1.4.1.18 S-(beta-aminoethyl)-L-cysteine + NAD+ 3% of the activity with L-lysine Agrobacterium tumefaciens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.18 dimer after preincubation with NAD+ without L-lysine Agrobacterium tumefaciens
1.4.1.18 tetramer gel filtration and centrifugation in presence of L-lysine Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.18 40
-
 stable for 10 min in absence of L-lysine, unstable above 40°C Agrobacterium tumefaciens
1.4.1.18 50
-
 stable for 10 min in presence of L-lysine, unstable above 50°C Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.18 9.7
-
-
 Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.18 3-acetylpyridine-NAD+ 16% of activity compared with NAD+ Agrobacterium tumefaciens
1.4.1.18 deamino-NAD+ 42% of activity compared with NAD+ Agrobacterium tumefaciens
1.4.1.18 NAD+
-
 Agrobacterium tumefaciens