Any feedback?
Literature summary extracted from
- Properties of meso-alpha,epsilon-diaminopimelate D-dehydrogenase from Bacillus sphaericus (1980), J. Biol. Chem., 255, 10599-10605.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.16 | D-2,6-diaminopimelate | - |
Lysinibacillus sphaericus |  |
| 1.4.1.16 | glyoxylate | 5 mM, 30% inhibition of reductive amination | Lysinibacillus sphaericus | |
| 1.4.1.16 | HgCl2 | 0.01 mM, complete inhibition | Lysinibacillus sphaericus | |
| 1.4.1.16 | L-2,6-diaminopimelate | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | L-alpha,epsilon-diaminopimelate | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | meso-alpha,delta-Diaminoadipate | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | p-chloromercuribenzoate | 0.001 mM, complete inhibition | Lysinibacillus sphaericus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.16 | 0.00083 | - |
NADP+ | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | 0.083 | 0.14 | NADP+ | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | 0.2 | - |
NADPH | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | 0.24 | - |
L-2-amino-6-oxopimelate | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | 2.5 | - |
meso-2,6-diaminopimelate | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | 12.5 | - |
NH3 | - |
Lysinibacillus sphaericus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.16 | 41000 | - |
2 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
| 1.4.1.16 | 78500 | 80000 | gel filtration | Lysinibacillus sphaericus |
| 1.4.1.16 | 78500 | 80000 | sedimentation equilibrium centrifugation | Lysinibacillus sphaericus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.16 | L-2-amino-6-oxoheptanedioate + NH3 + NADPH | Lysinibacillus sphaericus | - |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.16 | Lysinibacillus sphaericus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.16 | ammonium sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150 | Lysinibacillus sphaericus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.4.1.16 | meso-2,6-diaminoheptanedioate + H2O + NADP+ = L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | catalyzes the transfer of the pro-S hydrogen at C-4 of NADPH to the substrate, enzyme is B-stereospecific | Lysinibacillus sphaericus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.4.1.16 | -20°C, 0.01 M potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, 1 year | Lysinibacillus sphaericus |
| 1.4.1.16 | 4°C, 0.01% 2-mercaptoethanol, 20% glycerol, 1 month | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.16 | L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H | - |
Lysinibacillus sphaericus | meso-2,6-diaminoheptanedioate + H2O + NAD(P)+ | - |
r | |
| 1.4.1.16 | L-2-amino-6-oxoheptanedioate + NH3 + NADPH | - |
Lysinibacillus sphaericus | meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.1.16 | dimer | 2 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.16 | 45 | - |
unstable above | Lysinibacillus sphaericus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.16 | 7.5 | - |
2-amino-6-oxopimelate + NH3 + NADPH | Lysinibacillus sphaericus |
| 1.4.1.16 | 10.5 | - |
meso-2,6-diaminopimelate + H2O + NADP+ | Lysinibacillus sphaericus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.16 | 4.9 | 10 | 2-amino-6-oxopimelate + NH3 + NADPH | Lysinibacillus sphaericus |
| 1.4.1.16 | 6.8 | 11 | meso-2,6-diaminopimelate + H2O + NADP+ | Lysinibacillus sphaericus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.16 | 7 | - |
- |
Lysinibacillus sphaericus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.16 | NADPH | - |
Lysinibacillus sphaericus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.16 | 3.12 | - |
L-alpha,epsilon-diaminopimelate | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | 4 | - |
D-alpha,epsilon-diaminopimelate | - |
Lysinibacillus sphaericus | |
| 1.4.1.16 | 4.16 | - |
meso-alpha,delta-Diaminoadipate | - |
Lysinibacillus sphaericus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
