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Literature summary extracted from

  • Misono, H.; Ogasawara, M.; Nagasaki, S.
    Purification and properties of meso-diaminopimelate dehydrogenase from Brevibacterium sp. (1986), Agric. Biol. Chem., 50, 1329-1330.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.16 Cu2+ 0.01 mM, 68% inhibition Brevibacterium sp.
1.4.1.16 HgCl2 0.01 mM, 100% inhibition Brevibacterium sp.
1.4.1.16 iodoacetate 1 mM, 55% inhibition Brevibacterium sp.
1.4.1.16 N-ethylmaleimide 1 mM, 52% inhibition Brevibacterium sp.
1.4.1.16 Ni2+ 1 mM, 21% inhibition Brevibacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.16 0.083 0.14 NADP+
-
 Brevibacterium sp.
1.4.1.16 0.14
-
 NADP+
-
 Brevibacterium sp.
1.4.1.16 0.21
-
 L-2-amino-6-oxopimelate
-
 Brevibacterium sp.
1.4.1.16 0.23
-
 NADPH
-
 Brevibacterium sp.
1.4.1.16 2
-
 NAD+
-
 Brevibacterium sp.
1.4.1.16 2 10 NAD+
-
 Brevibacterium sp.
1.4.1.16 6.25
-
 meso-2,6-diaminopimelate
-
 Brevibacterium sp.
1.4.1.16 62.5
-
 NH3
-
 Brevibacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.16 39000
-
 2 * 39000, SDS-PAGE Brevibacterium sp.
1.4.1.16 70000
-
 gel filtration Brevibacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Brevibacterium sp.
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Brevibacterium sp. ICR 7000
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Brevibacterium sp.
-
-
-
1.4.1.16 Brevibacterium sp. ICR 7000
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.16
-
 Brevibacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.16 131
-
-
 Brevibacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Brevibacterium sp. meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Brevibacterium sp. ICR 7000 meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Brevibacterium sp. meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Brevibacterium sp. ICR 7000 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Brevibacterium sp. L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 r
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Brevibacterium sp. ICR 7000 L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.16 dimer 2 * 39000, SDS-PAGE Brevibacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.16 48
-
 10 min, 10 mM potassium phosphate pH 7.4, unstable above Brevibacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.16 8.5
-
 2-amino-6-oxopimelate + NH3 + NADPH Brevibacterium sp.
1.4.1.16 10.5
-
 meso-2,6-diaminopimelate + H2O + NADP+ Brevibacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.16 7 9 at 40°C Brevibacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.16 NADH 3% activity of that with NADP+ Brevibacterium sp.
1.4.1.16 NADPH
-
 Brevibacterium sp.