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Literature summary extracted from

  • Cordovilla, M.P.; Perez, J.; Ligero, F.; Lluch, C.; Valpuesta, V.
    Partial purification and characterization of NADH-glutamate synthase from faba bean (Vicia faba) root nodules (2000), Plant Sci., 150, 121-128.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.4.1.14 instability of enzyme during purification process Vicia faba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.14 3Fe-4S-center enzyme contains non-heme iron and acid-labile sulfur Vicia faba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.14 195000
-
 1 * 195000, SDS-PAGE Vicia faba
1.4.1.14 222000
-
 gel filtration Vicia faba
1.4.1.14 236000
-
 native PAGE Vicia faba

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.14 Vicia faba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.14 74fold partial purification Vicia faba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.14 additional information not found in crude soluble protein fractions from leaves or bacteroids Vicia faba
-
1.4.1.14 root nodule
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.14 2.66
-
-
 Vicia faba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH
-
 Vicia faba L-glutamate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.14 monomer 1 * 195000, SDS-PAGE Vicia faba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.14 30
-
 assay at Vicia faba

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.14 flavin flavoprotein Vicia faba
1.4.1.14 NADH
-
 Vicia faba