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Literature summary extracted from

  • Marquez, A.J.; Galvan, F.; Vega, J.M.
    Purification and characterization of the NADH-glutamate synthase from Chlamydomonas reinhardii (1984), Plant Sci. Lett., 34, 305-314.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.14 6-diazo-5-oxo-L-norleucine 76% inhibition at 5 mM Chlamydomonas reinhardtii
1.4.1.14 acridine inhibitor of flavoenzymes, 65% inhibition Chlamydomonas reinhardtii
1.4.1.14 azaserine 75% inhibition at 5 mM Chlamydomonas reinhardtii
1.4.1.14 bathophenanthroline 50% inhibition Chlamydomonas reinhardtii
1.4.1.14 additional information not inhibited by 1 mM cyanide or cyanate Chlamydomonas reinhardtii
1.4.1.14 p-hydroxymercuribenzoate complete inhibition at 0.1-1 mM, at 0.0005 mM 33% inhibition or 75% inhibition after preincubation in presence of glutamine and 2-oxoglutarate, substrates induce a conformation, which makes essential sulfhydryl groups more accessible to reagent Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.14 additional information
-
 additional information
-
 Chlamydomonas reinhardtii
1.4.1.14 0.0076
-
 NADH
-
 Chlamydomonas reinhardtii
1.4.1.14 0.0182
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii
1.4.1.14 0.6
-
 L-glutamine
-
 Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.14 Chlamydomonas reinhardtii
-
-
-
1.4.1.14 Chlamydomonas reinhardtii 21 gr
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.4.1.14 4°C, a few days under air, stable Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.14 350fold purification Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.14 4.56
-
-
 Chlamydomonas reinhardtii

Storage Stability

EC Number Storage Stability Organism
1.4.1.14 4°C, a few days under air, stable Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Chlamydomonas reinhardtii L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Chlamydomonas reinhardtii L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Chlamydomonas reinhardtii 21 gr L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Chlamydomonas reinhardtii 21 gr L-glutamate + NAD+
-
 ?
1.4.1.14 additional information no activity with hydroxylamine Chlamydomonas reinhardtii ?
-
 ?
1.4.1.14 additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Chlamydomonas reinhardtii ?
-
 ?
1.4.1.14 additional information no activity with hydroxylamine Chlamydomonas reinhardtii 21 gr ?
-
 ?
1.4.1.14 additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Chlamydomonas reinhardtii 21 gr ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.14 45
-
 10 min, 86% loss of activity Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.14 NADH specific for NADH as reductant Chlamydomonas reinhardtii