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Literature summary extracted from

  • Khanna, S.; Nicholas, D.J.D.
    Some properties of glutamine synthetase and glutamate synthase from Chlorobium vibrioforme f. thiosulfatophilum (1983), Arch. Microbiol., 134, 98-103.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.14 diphosphate 5 mM, stimulates activity with 36% enhancement Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 succinate 50 mM, stimulates activity by 57% Prosthecochloris vibrioformis f. thiosulfatophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.14 ADP 20 mM, about 75% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 alanine slight inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 AMP 20 mM, about 75% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 arginine 20 mM, complete inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 Atebrin
-
 Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 ATP 5 mM, 75% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 glycine 48% inhibition at 10 mM, complete inhibition at 20 mM Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 leucine 10 mM, 52% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 malate 20 mM, 30% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 methionine 10 mM, 72% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 additional information cumulative inhibition by combinations of amino acids and nucleotides, cumulative feedback inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 NAD+ 1 mM, 27% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 NADP+ 1 mM, 48% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 phenylalanine 20 mM, complete inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 proline
-
 Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 pyruvate 20 mM, 20% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 serine 10 mM, 73% inhibition Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 tryptophan 29% inhibition at 10 mM, complete inhibition at 20 mM Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 valine
-
 Prosthecochloris vibrioformis f. thiosulfatophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.14 0.013
-
 NADH
-
 Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 0.27
-
 2-oxoglutarate
-
 Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 0.769
-
 L-glutamine
-
 Prosthecochloris vibrioformis f. thiosulfatophilum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.14 chromatophore
-
 Prosthecochloris vibrioformis f. thiosulfatophilum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Prosthecochloris vibrioformis f. thiosulfatophilum glutamine synthetase/glutamate synthase pathway for ammonia assimilation is fafavoured by growth at low concentrations of ammonia glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Prosthecochloris vibrioformis f. thiosulfatophilum key enzyme for glutamate production glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 glutamine synthetase/glutamate synthase pathway for ammonia assimilation is fafavoured by growth at low concentrations of ammonia glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 key enzyme for glutamate production glutamate + NAD+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.14 Prosthecochloris vibrioformis f. thiosulfatophilum
-
-
-
1.4.1.14 Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.14 222fold purification Prosthecochloris vibrioformis f. thiosulfatophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.14 4.7
-
-
 Prosthecochloris vibrioformis f. thiosulfatophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Prosthecochloris vibrioformis f. thiosulfatophilum L-glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Prosthecochloris vibrioformis f. thiosulfatophilum L-glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 L-glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 L-glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH glutamine synthetase/glutamate synthase pathway for ammonia assimilation is fafavoured by growth at low concentrations of ammonia Prosthecochloris vibrioformis f. thiosulfatophilum glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH key enzyme for glutamate production Prosthecochloris vibrioformis f. thiosulfatophilum glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH glutamine synthetase/glutamate synthase pathway for ammonia assimilation is fafavoured by growth at low concentrations of ammonia Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH key enzyme for glutamate production Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 glutamate + NAD+
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.14 30
-
 assay at Prosthecochloris vibrioformis f. thiosulfatophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.14 flavin flavin component Prosthecochloris vibrioformis f. thiosulfatophilum
1.4.1.14 NADH specific for NADH as reductant Prosthecochloris vibrioformis f. thiosulfatophilum