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Literature summary extracted from

  • Hayakawa, T.; Yamaya, T.; Kamachi, K.; Ojima, K.
    Purification, characterization, and immunological properties of NADH-dependent glutamate synthase from rice cell cultures (1992), Plant Physiol., 98, 1317-1322.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.14 azaserine completely inhibited by 4 mM Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.14 0.003
-
 NADH
-
 Oryza sativa
1.4.1.14 0.076
-
 2-oxoglutarate
-
 Oryza sativa
1.4.1.14 0.811
-
 L-glutamine
-
 Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.14 194000
-
 gel filtration Oryza sativa
1.4.1.14 196000
-
 1 * 196000, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Oryza sativa role in synthesis of glutamate for growth and development in higher plants glutamate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.14 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.14 815fold purification Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.14 cell culture
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.14 9.8
-
-
 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Oryza sativa L-glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Oryza sativa L-glutamate + NAD+
-
 ir
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH role in synthesis of glutamate for growth and development in higher plants Oryza sativa glutamate + NAD+
-
 ?
1.4.1.14 additional information no activity with L-asparagine, NH4Cl or NADPH Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.14 monomer 1 * 196000, SDS-PAGE Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.14 30
-
 assay at Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.14 32
-
 L-glutamine
-
 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.14 flavin flavoprotein Oryza sativa
1.4.1.14 additional information no activity with NADPH Oryza sativa
1.4.1.14 NADH specific for NADH as reductant Oryza sativa