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Literature summary extracted from

  • Vanoni, M.A.; Fischer, F.; Ravasio, S.; Verzotti, E.; Edmondson, D.E.; Hagen, W.R.; Zanetti, G.; Curti, B.
    The recombinant alpha subunit of glutamate synthase: spectroscopic and catalytic properties (1998), Biochemistry, 37, 1828-1838.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.13 production of the alpha and beta subunits in Escherichia coli Azospirillum brasilense

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 3-Aminopyridine adenine dinucleotide phosphate good inhibitor of the holoenzyme, competitive with respect to NADPH Azospirillum brasilense
1.4.1.13 iodonitrotetrazolium inhibitor of the L-glutamate:iodonitrotetrazolim oxidoreductase activity of the alpha subunit at concentrations above 0.1 mM Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 of the alpha subunit, using column chromatography on Q-Sepharose and Ultrogel AcA34 Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Azospirillum brasilense L-glutamate + NADP+
-
 ?
1.4.1.13 additional information the alpha subunit catalyzes the synthesis of glutamate from L-glutamine and 2-oxoglutarate, provided that a reducing system is present, reducing system: dithionite and methyl viologen Azospirillum brasilense ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 flavin the alpha subunit contains FMN as flavin cofactor, 0.94 FMN bound per alpha subunit Azospirillum brasilense
1.4.1.13 flavin the beta subunit contains the binding site for FAD, 0.83 mol FAD per mol beta subunit Azospirillum brasilense
1.4.1.13 additional information the alpha subunit contains the [3Fe-4S] cluster of the enzyme Azospirillum brasilense
1.4.1.13 NADPH the beta subunit contains the NADPH binding site of the enzyme Azospirillum brasilense

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.13 additional information
-
 additional information
-
 Azospirillum brasilense
1.4.1.13 0.0024
-
 3-Aminopyridine adenine dinucleotide phosphate inhibitor of the holoenzyme, competitive with NADPH Azospirillum brasilense