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Literature summary extracted from

  • Vanoni, M.A.; Verzotti, E.; Zanetti, G.; Curti, B.
    Properties of the recombinant beta subunit of glutamate synthase (1996), Eur. J. Biochem., 236, 937-946.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.13 production of the beta subunit in Escherichia coli Azospirillum brasilense

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, uncompetitive inhibition with ferricyanide or iodonitrotetrazolium as substrate, competitive inhibition with NADPH as substrate Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.0035
-
 NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: iodonitrotetrazolium Azospirillum brasilense
1.4.1.13 0.0098
-
 NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: menadione Azospirillum brasilense
1.4.1.13 0.0118
-
 NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: ferricyanide Azospirillum brasilense
1.4.1.13 0.035
-
 menadione of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme Azospirillum brasilense
1.4.1.13 0.05
-
 iodonitrotetrazolium of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme Azospirillum brasilense
1.4.1.13 0.14
-
 ferricyanide of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme Azospirillum brasilense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.13 Iron iron-sulfur protein Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 of the recombinant enzyme beta subunit, using ammonium sulfate fractionation, affinity chromatography on Reactive Red, ultrafiltration and column chromatography on Ultrogel AcA 54 Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 ferricyanide + NADPH + H+
-
 Azospirillum brasilense ferrocyanide + NADP+
-
 r
1.4.1.13 iodonitrotetrazolium + NADPH + H+
-
 Azospirillum brasilense reduced iodonitrotetrazolium + NADP+
-
 r
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Azospirillum brasilense L-glutamate + NADP+
-
 ?
1.4.1.13 menadione + NADPH + H+
-
 Azospirillum brasilense menadiol + NADP+
-
 r
1.4.1.13 additional information the enzyme beta subunit is devoid of glutamate synthase activity in either direction at both pH 7.5 and 9.5, but it can oxidize NADPH and transfer electrons to synthetic electron acceptors like iodonitrotetrazolium, ferricyanide, menadione, dichloroindophenol, the beta subunit is highly specific toward NADPH, the rate of oxidation of NADH in the presence of electron acceptors is less than 5% of that measured with NADPH Azospirillum brasilense ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.13 18.3
-
 NADPH of NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, co-substrate: iodonitrotetrazolium, inhibitor: 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose Azospirillum brasilense
1.4.1.13 20
-
 iodonitrotetrazolium of NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, co-substrate: NADPH, inhibitor: 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose Azospirillum brasilense
1.4.1.13 25.3
-
 menadione of NADPH:acceptor oxidoreductase acitivity of the beta subunit of the enzyme Azospirillum brasilense
1.4.1.13 30.8
-
 NADPH of NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, co-substrate: ferricyanide, inhibitor: 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose Azospirillum brasilense
1.4.1.13 32
-
 iodonitrotetrazolium of NADPH:acceptor oxidoreductase acitivity of the beta subunit of the enzyme Azospirillum brasilense
1.4.1.13 39.5
-
 ferricyanide of NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, co-substrate: NADPH, inhibitor: 2'-phosphoadenosine-5'-diphospho-5'beta-D-ribose Azospirillum brasilense
1.4.1.13 41.9
-
 ferricyanide of NADPH:acceptor oxidoreductase acitivity of the beta subunit of the enzyme Azospirillum brasilense

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 flavin flavoenzyme Azospirillum brasilense
1.4.1.13 flavin the beta subunit contains the binding site for FAD, 0.83 mol FAD per mol beta subunit Azospirillum brasilense
1.4.1.13 additional information the enzyme contains three distinct ion-sulfur centers per alphabeta protomer Azospirillum brasilense
1.4.1.13 NADPH the beta subunit contains the NADPH binding site of the enzyme Azospirillum brasilense

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.13 0.021
-
 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, substrate: NADPH, cosubstrate: ferricyanide Azospirillum brasilense
1.4.1.13 0.08
-
 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, substrate: NADPH, cosubstrate: iodonitrotetrazolium Azospirillum brasilense
1.4.1.13 0.18
-
 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, substrate: ferricyanide, cosubstrate: NADPH Azospirillum brasilense
1.4.1.13 0.495
-
 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, substrate: iodonitrotetrazolium, cosubstrate: NADPH Azospirillum brasilense