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Literature summary extracted from

  • Meister, A.
    Glutamate synthase from Escherichia coli, Klebsiella aerogenes, and Saccharomyces cerevisiae (1985), Methods Enzymol., 113, 327-337.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.14 2-mercaptoethanol stabilizes Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 L-2-Amino-4-oxo-5-chloropentanoic acid selective inhibitor of glutamine-dependent activity Escherichia coli
1.4.1.13 L-2-Amino-4-oxo-5-chloropentanoic acid selective inhibitor of glutamine-dependent activity Klebsiella aerogenes
1.4.1.14 (2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid
-
 Saccharomyces cerevisiae
1.4.1.14 D-glutamine
-
 Saccharomyces cerevisiae
1.4.1.14 DL-ethionine sulfone
-
 Saccharomyces cerevisiae
1.4.1.14 DL-homocysteic acid
-
 Saccharomyces cerevisiae
1.4.1.14 L-Albizziin
-
 Saccharomyces cerevisiae
1.4.1.14 L-homocysteine sulfonamide very potent transition state inhibitor, competitive to L-glutamine Saccharomyces cerevisiae
1.4.1.14 L-methionine sulfone markedly inhibits Saccharomyces cerevisiae
1.4.1.14 L-methionine-SR-sulfoxide
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.012
-
 NADPH
-
 Klebsiella aerogenes
1.4.1.13 0.3
-
 L-glutamine
-
 Klebsiella aerogenes
1.4.1.13 0.3
-
 alpha-ketoglutarate
-
 Klebsiella aerogenes
1.4.1.13 0.5 2 NH4Cl
-
 Klebsiella aerogenes
1.4.1.14 0.007
-
 NADH
-
 Saccharomyces cerevisiae
1.4.1.14 0.04
-
 2-oxoglutarate
-
 Saccharomyces cerevisiae
1.4.1.14 0.28
-
 L-glutamine
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.13 51500
-
 x * 175000 + x * 51500, SDS-PAGE Klebsiella aerogenes
1.4.1.13 175000
-
 x * 175000 + x * 51500, SDS-PAGE Klebsiella aerogenes
1.4.1.14 61000
-
 1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol Saccharomyces cerevisiae
1.4.1.14 169000
-
 1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol Saccharomyces cerevisiae
1.4.1.14 265000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Escherichia coli
-
-
-
1.4.1.13 Klebsiella aerogenes
-
-
-
1.4.1.14 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using sonication, heat treatment, protamine sulfate precipitation, ammonium sulfate precipitation, gel filtration on G-50, column chromatography on DEAE-Sephadex and gel filtration on Sephadex G-200 and Sepharose 6B Klebsiella aerogenes
1.4.1.14
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 additional information
-
-
 Escherichia coli
1.4.1.13 additional information
-
-
 Klebsiella aerogenes
1.4.1.13 13.33
-
-
 Escherichia coli
1.4.1.13 20.83
-
-
 Klebsiella aerogenes
1.4.1.14 41.7
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.4.1.13 4°C, 200 mM potassium phosphate buffer, pH 7.6, 0.5 mM Na2EDTA Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ in the reverse reaction ammonia can act instead of glutamine, but more slowly Escherichia coli L-glutamate + NADP+
-
 ?
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ in the reverse reaction ammonia can act instead of glutamine, but more slowly Klebsiella aerogenes L-glutamate + NADP+
-
 ?
1.4.1.13 additional information
-
 Escherichia coli ?
-
 ?
1.4.1.13 additional information glutamine binding site of the enzyme is located on the heavy subunit of the enzyme, preparations of the enzyme that lack flavins or the flavins and iron sulfide catalyze NH3-dependent reaction but not glutamine-dependent reaction Klebsiella aerogenes ?
-
 ?
1.4.1.13 NH3 + 2-oxoglutarate + NADPH + H+
-
 Escherichia coli L-glutamate + NADP+
-
 ?
1.4.1.13 NH3 + 2-oxoglutarate + NADPH + H+
-
 Klebsiella aerogenes L-glutamate + NADP+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH low activity when NH3 is substituted for glutamine, NH4Cl: 2.7% of the activity with L-glutamine Saccharomyces cerevisiae L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Saccharomyces cerevisiae L-glutamate + NAD+
-
 ?
1.4.1.14 additional information no activity with 2-oxovalerate, 2-oxopimelate, 2-oxoadipate, oxaloacetate, D-glutamine, L- or D-asparagine, L- or D-homoglutamine, alpha-methyl-L-glutamine, DL-beta-glutamine, L-isoglutamine, glutaramide or glutaramate Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.13 ? x * 175000 + x * 51500, SDS-PAGE Klebsiella aerogenes
1.4.1.14 dimer 1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.13 7.8
-
 glutamine-dependent activity Klebsiella aerogenes
1.4.1.13 8
-
 glutamine-dependent activity Klebsiella aerogenes
1.4.1.13 9
-
 ammonia-dependent activity Klebsiella aerogenes
1.4.1.14 7 7.5 glutamine-dependent NADH oxidase activity Saccharomyces cerevisiae
1.4.1.14 9
-
 ammonia-dependent NADH oxidase activity, rate steadily increases from pH 6 to 9 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 NADH no activity with NADH Escherichia coli
1.4.1.13 NADH no activity with NADH Klebsiella aerogenes
1.4.1.13 NADPH
-
 Escherichia coli
1.4.1.13 NADPH
-
 Klebsiella aerogenes
1.4.1.14 additional information no activity with NADPH Saccharomyces cerevisiae
1.4.1.14 NADH specific for NADH as reductant Saccharomyces cerevisiae