EC Number | General Stability | Organism |
---|---|---|
1.4.1.13 | alpha-ketoglutarate stabilizes | [Brevibacterium] flavum |
1.4.1.13 | glutamine stabilizes | [Brevibacterium] flavum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | 2'-adenylic acid | - |
[Brevibacterium] flavum | |
1.4.1.13 | AgNO3 | at 0.001 mM completely inhibits | [Brevibacterium] flavum | |
1.4.1.13 | ATP | at 3.5 mM 12% inhibition | [Brevibacterium] flavum | |
1.4.1.13 | D-glutamate | at 3.5 mM 10% inhibition | [Brevibacterium] flavum | |
1.4.1.13 | D-Lysine | significantly inhibits | [Brevibacterium] flavum | |
1.4.1.13 | HgCl2 | at 0.001 mM remains 8% of activity | [Brevibacterium] flavum | |
1.4.1.13 | iodoacetate | completely inhibits at 1 mM, 2-mercaptoethanol protects against inhibition | [Brevibacterium] flavum | |
1.4.1.13 | L-glutamate | at 3.5 mM 13% inhibition | [Brevibacterium] flavum | |
1.4.1.13 | L-histidine | significant inhibitor | [Brevibacterium] flavum | |
1.4.1.13 | L-homoserine | significant inhibitor | [Brevibacterium] flavum | |
1.4.1.13 | L-malate | at 3.5 mM 67% inhibition | [Brevibacterium] flavum | |
1.4.1.13 | L-methionine | - |
[Brevibacterium] flavum | |
1.4.1.13 | lysine | significantly inhibits | [Brevibacterium] flavum | |
1.4.1.13 | methionine sulfone | - |
[Brevibacterium] flavum | |
1.4.1.13 | NADH | at 3.5 mM 31% inhibition | [Brevibacterium] flavum | |
1.4.1.13 | NADP+ | 76% inhibition | [Brevibacterium] flavum | |
1.4.1.13 | oxalglycine | - |
[Brevibacterium] flavum | |
1.4.1.13 | p-chloromercuribenzoate | completely inhibits at 1 mM, 2-mercaptoethanol protects against inhibition | [Brevibacterium] flavum | |
1.4.1.13 | phenylalanine | significantly inhibits | [Brevibacterium] flavum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.13 | 0.06 | - |
NADPH | - |
[Brevibacterium] flavum | |
1.4.1.13 | 0.06 | - |
alpha-ketoglutarate | - |
[Brevibacterium] flavum | |
1.4.1.13 | 0.24 | - |
L-glutamine | - |
[Brevibacterium] flavum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | Iron | enzyme contains 8 mol of non-heme iron | [Brevibacterium] flavum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.13 | [Brevibacterium] flavum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.13 | using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, Sepharose 6B, hydroxyapatite, glutamate dehydrogenase antibody affinity chromatography and column chromatography on Sephacryl S-200 | [Brevibacterium] flavum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.4.1.13 | 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+ | hexa-uni ping pong mechanism | [Brevibacterium] flavum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 30.5 | - |
- |
[Brevibacterium] flavum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | highly specific | [Brevibacterium] flavum | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | glyoxylate shows 3% reactivity compared with alpha-ketoglutarate | [Brevibacterium] flavum | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | ammonia does not replace L-glutamine as amino donor | [Brevibacterium] flavum | L-glutamate + NADP+ | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 7 | - |
- |
[Brevibacterium] flavum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | flavin | enzyme contains 2 mol of FMN and 2 mol of FAD | [Brevibacterium] flavum | |
1.4.1.13 | NADH | no activity with NADH | [Brevibacterium] flavum | |
1.4.1.13 | NADPH | - |
[Brevibacterium] flavum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.13 | 0.01 | - |
methionine sulfone | competitive inhibitor with respect to glutamine | [Brevibacterium] flavum | |
1.4.1.13 | 0.041 | - |
methionine sulfone | uncompetitive inhibitor with respect to alpha-ketoglutarate | [Brevibacterium] flavum | |
1.4.1.13 | 0.045 | - |
methionine sulfone | uncompetitive inhibitor with respect to NADPH | [Brevibacterium] flavum | |
1.4.1.13 | 0.25 | - |
NADP | competitive inhibitor vs. NADPH | [Brevibacterium] flavum | |
1.4.1.13 | 0.69 | - |
NADP | uncompetitive inhibitor vs. alpha-ketoglutarate | [Brevibacterium] flavum | |
1.4.1.13 | 0.8 | - |
oxalylglycine | competitive inhibitor with respect to alpha-ketoglutarate | [Brevibacterium] flavum | |
1.4.1.13 | 1.13 | - |
NADP | uncompetitive inhibitor vs. glutamine | [Brevibacterium] flavum | |
1.4.1.13 | 2.1 | - |
2'-adenylic acid | competitive inhibitor with respect to NADPH | [Brevibacterium] flavum | |
1.4.1.13 | 17.1 | - |
2'-adenylic acid | uncompetitive inhibitor with respect to glutamine | [Brevibacterium] flavum | |
1.4.1.13 | 17.2 | - |
2'-adenylic acid | uncompetitive inhibitor with respect to alpha-keto-glutarate | [Brevibacterium] flavum | |
1.4.1.13 | 19.3 | - |
oxalylglycine | uncompetitive inhibitor with respect to glutamine | [Brevibacterium] flavum | |
1.4.1.13 | 20.6 | - |
oxalylglycine | uncompetitive inhibitor with respect to NADP | [Brevibacterium] flavum | |
1.4.1.13 | 25 | - |
glutamate | competitive inhibitor with respect to glutamine | [Brevibacterium] flavum | |
1.4.1.13 | 45 | - |
glutamate | competitive inhibitor with respect to alpha-ketoglutarate | [Brevibacterium] flavum |