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Literature summary extracted from

  • Ratti, S.; Curti, B.; Zanetti, G.; Galli, E.
    Purification and characterization of glutamate synthase from Azospirillum brasilense (1985), J. Bacteriol., 163, 724-729.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 6-diazo-5-oxo-L-norleucine potent inhibitor, complete loss of activity at 0.1 mM after 10 min of preincubation Azospirillum brasilense
1.4.1.13 CaCl2
-
 Azospirillum brasilense
1.4.1.13 KBr
-
 Azospirillum brasilense
1.4.1.13 KI
-
 Azospirillum brasilense
1.4.1.13 L-asparagine activity decreases to about 0.5% of the original at 25 mM Azospirillum brasilense
1.4.1.13 L-methionine competitive inhibitor with respect to L-glutamine Azospirillum brasilense
1.4.1.13 NaClO4
-
 Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.00625
-
 NADPH
-
 Azospirillum brasilense
1.4.1.13 0.029
-
 2-oxoglutarate
-
 Azospirillum brasilense
1.4.1.13 0.45
-
 L-glutamine
-
 Azospirillum brasilense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.13 Iron iron-sulfur protein Azospirillum brasilense
1.4.1.13 Iron 8.1 iron atoms per MW 185000 Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.13 50000
-
 4 * 135000 + 4 * 50000, SDS-PAGE Azospirillum brasilense
1.4.1.13 135000
-
 4 * 135000 + 4 * 50000, SDS-PAGE Azospirillum brasilense
1.4.1.13 740000
-
 gel filtration Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using heat treatment, ammonium sulfate precipitation, column chromatography on DEAE-Trisacryl, gel filtration and affinity chromatogray Azospirillum brasilense

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 19.5
-
-
 Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ when L-glutamine is replaced by ammonia as the amino-group donor, the catalytic activity is less than 1% Azospirillum brasilense L-glutamate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.13 octamer 4 * 135000 + 4 * 50000, SDS-PAGE Azospirillum brasilense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.13 7.5
-
-
 Azospirillum brasilense

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.13 7.3 8.3
-
 Azospirillum brasilense

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 flavin flavoenzyme Azospirillum brasilense
1.4.1.13 flavin contains FAD and FMN Azospirillum brasilense
1.4.1.13 flavin ratio FAD: FMN is 1 Azospirillum brasilense
1.4.1.13 additional information the enzyme contains 7.9 sulfur atoms per protomer with a molecular weight of 185000 Azospirillum brasilense
1.4.1.13 NADH no activity with NADH Azospirillum brasilense
1.4.1.13 NADPH
-
 Azospirillum brasilense