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Literature summary extracted from

  • Mei, B.; Jiao, R.
    Purification and properties of glutamate synthase from Nocardia mediterranei (1988), J. Bacteriol., 170, 1940-1944.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.4.1.13 2 mM alpha-ketoglutarate, 10 mM beta-mercaptoethanol and 10% glycerol when present together markedly enhance stability Amycolatopsis mediterranei
1.4.1.13 unprotected enzyme is unstable, losing 65% of its activity in 24 hours at 4°C Amycolatopsis mediterranei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 D-glutamate
-
Amycolatopsis mediterranei
1.4.1.13 glycine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
1.4.1.13 isocitrate 35% inhibition at 5 mM Amycolatopsis mediterranei
1.4.1.13 L-alanine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
1.4.1.13 L-asparagine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
1.4.1.13 L-glutamate potent inhibitor Amycolatopsis mediterranei
1.4.1.13 L-histidine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
1.4.1.13 L-leucine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
1.4.1.13 L-malate more than 50% inhibition at 5 mM Amycolatopsis mediterranei
1.4.1.13 L-methionine shows no effect Amycolatopsis mediterranei
1.4.1.13 L-serine less than 50% inhibition at 5 mM Amycolatopsis mediterranei
1.4.1.13 L-tryptophan strong inhibitor Amycolatopsis mediterranei
1.4.1.13 NADP+ potent inhibitor Amycolatopsis mediterranei
1.4.1.13 succinate 25% inhibition at 5 mM Amycolatopsis mediterranei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.053
-
alpha-ketoglutarate
-
Amycolatopsis mediterranei
1.4.1.13 0.077
-
L-glutamine
-
Amycolatopsis mediterranei
1.4.1.13 0.11
-
NADPH
-
Amycolatopsis mediterranei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.13 Iron iron-sulfur protein Amycolatopsis mediterranei
1.4.1.13 Iron contains 7.5 mol of nonheme iron Amycolatopsis mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.13 55000
-
1 * 145000 + 1 * 55000, SDS-PAGE Amycolatopsis mediterranei
1.4.1.13 145000
-
1 * 145000 + 1 * 55000, SDS-PAGE Amycolatopsis mediterranei
1.4.1.13 195000
-
sucrose density gradient centrifugation Amycolatopsis mediterranei

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Amycolatopsis mediterranei
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.4.1.13 presence of reducing agents is essential for stabilization Amycolatopsis mediterranei

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using protamine sulfate precipitation, ammonium sulfate precipitation, column chromatography on DE52, DEAE-Sephadex, hydroxyapatite, phenyl-Sepharose CL-4B and Bio-Gel filtration Amycolatopsis mediterranei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 1.48
-
-
Amycolatopsis mediterranei

Storage Stability

EC Number Storage Stability Organism
1.4.1.13 4°C, unprotected enzyme loses 65% of activity after 24 h Amycolatopsis mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ in the reverse reaction ammonia can act instead of glutamine, but more slowly Amycolatopsis mediterranei L-glutamate + NADP+
-
?
1.4.1.13 NH3 + 2-oxoglutarate + NADPH + H+ 14% relative activity to L-glutamine Amycolatopsis mediterranei L-glutamate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.4.1.13 dimer 1 * 145000 + 1 * 55000, SDS-PAGE Amycolatopsis mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.13 7.5 8.5 broad optimum Amycolatopsis mediterranei

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 flavin contains FAD and FMN Amycolatopsis mediterranei
1.4.1.13 flavin ratio FAD: FMN is 1.1 Amycolatopsis mediterranei
1.4.1.13 additional information the enzyme contains 7.2 mol of acid-labile sulfur per 200,000 g of protein Amycolatopsis mediterranei
1.4.1.13 NADH no activity with NADH Amycolatopsis mediterranei
1.4.1.13 NADPH
-
Amycolatopsis mediterranei