EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.99.5 | expression in Escherichia coli JM109 carrying pXE25, activity is maximal 18times higher than the activity expressed by induced cells of Comamonas testosteroni. Overexpression in Pseudomonas putida KT2440 using pMMB208 as expression vector, activity is maximaly 264times higher than the activity expressed by induced cells of Comamonas testosteroni | Comamonas testosteroni |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.99.5 | 0.0066 | - |
1-Androstene-3,17-dione | reaction with N,N,N,N-tetramethyl-1,4-phenylenediamine | Comamonas testosteroni | |
1.3.99.5 | 0.0161 | - |
5alpha-androstane-3,17-dione | reaction with N,N,N,N-tetramethyl-1,4-phenylenediamine | Comamonas testosteroni |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.99.5 | 58100 | - |
x * 58100, calculation from nucleotide sequence | Comamonas testosteroni |
1.3.99.5 | 60000 | - |
x * 60000, SDS-PAGE | Comamonas testosteroni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.5 | testosterone + reduced acceptor | Comamonas testosteroni | key enzyme in the degradation of steroids having an A:B ring fusion in a trans configuration | 5alpha-dihydrotestosterone + acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.99.5 | Comamonas testosteroni | - |
formerly Pseudomonas testosteroni | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.5 | 1-androstene-3,17-dione + phenazine methosulfate | acceptor: N,N,N,N-tetramethyl-1,4-phenylenediamine, menadione, phenazine methosulfate, cytochrome c | Comamonas testosteroni | 1,4-androstadiene-3,17-dione + reduced phenazine methosulfate | - |
ir | |
1.3.99.5 | 5alpha-1-androsten-3,17-dione + acceptor | acceptor: N,N,N,N-tetramethyl-1,4-phenylenediamine, menadione, phenazine methosulfate, cytochrome c | Comamonas testosteroni | ? + reduced acceptor | - |
ir | |
1.3.99.5 | 5alpha-androstane-3,17-dione + N,N,N',N'-tetramethyl-1,4-phenylendiamine | - |
Comamonas testosteroni | androst-4-ene-3,17-dione + reduced N,N,N',N'-tetramethyl-1,4-phenylendiamine | - |
r | |
1.3.99.5 | testosterone + reduced acceptor | key enzyme in the degradation of steroids having an A:B ring fusion in a trans configuration | Comamonas testosteroni | 5alpha-dihydrotestosterone + acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.99.5 | ? | x * 60000, SDS-PAGE | Comamonas testosteroni |
1.3.99.5 | ? | x * 58100, calculation from nucleotide sequence | Comamonas testosteroni |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.99.5 | 40 | - |
reaction with 1-androstene-3,17-dione and N,N,N,N-tetramethyl-1,4-phenylenediamine | Comamonas testosteroni |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.99.5 | 25 | 50 | 25°C: about 50% of maximal activity, 50°C: about 20% of maximal activity, reaction with N,N,N,N-tetramethyl-1,4-phenylenediamine | Comamonas testosteroni |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.99.5 | 8 | 9 | reaction with 1-androstene-3,17-dione and N,N,N,N-tetramethyl-1,4-phenylenediamine | Comamonas testosteroni |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.99.5 | 7 | 9 | pH 7.0: about 40% of maximal activity, pH 8.0-9.0: maximal activity, reaction with Delta1-androstene-3,17-dione and N,N,N,N-tetramethyl-1,4-phenylenediamine | Comamonas testosteroni |