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Literature summary extracted from

  • Morii, S.; Fujii, C.; Miyoshi, T.; Iwami, M.; Itagaki, E.
    3-Ketosteroid-DELTA1-dehydrogenase of Rhodococcus rhodochrous: sequencing of the genomic DNA and hyperexpression, purification, and characterization of the recombinant enzyme (1998), J. Biochem., 124, 1026-1032.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.4 expression in Escherichia coli Rhodococcus rhodochrous

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.4 0.045
-
 4-androstene-3,17-dione
-
 Rhodococcus rhodochrous

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.99.4 membrane partly, recombinant protein in Escherichia coli Rhodococcus rhodochrous 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.4 55000
-
 SDS-PAGE, recombinant protein, expression in Escherichia coli Rhodococcus rhodochrous

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.4 recombinant protein expressed in Escherichia coli Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + phenazine methosulfate
-
 Rhodococcus rhodochrous 1,4-androstadiene-3,17-dione + reduced phenazine methosulfate
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.4 flavin absorption maxima at 276, 362, 458 nm Rhodococcus rhodochrous