Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Levy, H.R.; Talalay, P.
    Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation (1959), J. Biol. Chem., 234, 2014-2021.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.4 CuSO4
-
 Comamonas testosteroni
1.3.99.4 estrone slight Comamonas testosteroni
1.3.99.4 HgCl2
-
 Comamonas testosteroni
1.3.99.4 MnSO4
-
 Comamonas testosteroni
1.3.99.4 o-Iodosobenzoate
-
 Comamonas testosteroni
1.3.99.4 p-chloromercuribenzoate
-
 Comamonas testosteroni
1.3.99.4 p-chloromercuriphenylsulfonate
-
 Comamonas testosteroni
1.3.99.4 ZnSO4
-
 Comamonas testosteroni
1.3.99.5 1,10-phenanthroline 1 mM, 29% inhibition Comamonas testosteroni
1.3.99.5 1,4-androstadiene-3,17-dione
-
 Comamonas testosteroni
1.3.99.5 2,2'-dipyridyl 1 mM, 22% inhibition Comamonas testosteroni
1.3.99.5 8-hydroxyquinoline 1 mM, 10% inhibition Comamonas testosteroni
1.3.99.5 CuSO4 1 mM, 95% inhibition Comamonas testosteroni
1.3.99.5 EDTA 1 mM, 23% inhibition Comamonas testosteroni
1.3.99.5 estrone
-
 Comamonas testosteroni
1.3.99.5 HgCl2 1 mM, complete inhibition Comamonas testosteroni
1.3.99.5 iodoacetamide 1 mM, 11% inhibition Comamonas testosteroni
1.3.99.5 iodoacetate 1 mM, 8% inhibition Comamonas testosteroni
1.3.99.5 KCN 1 mM, 24% inhibition Comamonas testosteroni
1.3.99.5 MnSO4 1 mM, 42% inhibition Comamonas testosteroni
1.3.99.5 NaN3 1 mM, 26% inhibition Comamonas testosteroni
1.3.99.5 o-Iodosobenzoate 1 mM, complete inhibition Comamonas testosteroni
1.3.99.5 p-chloromercuribenzoate 1 mM, 43% inhibition Comamonas testosteroni
1.3.99.5 ZnSO4 1 mM, 53% inhibition Comamonas testosteroni

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.99.4 particle-bound associated with fine particulate structures Comamonas testosteroni
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Comamonas testosteroni
-
-
-
1.3.99.5 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.4
-
 Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 11alpha-hydroxy-testosterone + acceptor
-
 Comamonas testosteroni 11alpha,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor
-
 ?
1.3.99.4 11beta-hydroxy-4-androstene-3,17-dione + acceptor
-
 Comamonas testosteroni 11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
-
 ?
1.3.99.4 17alpha-hydroxy-progesterone + acceptor 4-pregnene-17alpha-ol-3,20-dione Comamonas testosteroni 1,4-pregnene-17alpha-ol-3,20-dione + reduced acceptor
-
 ?
1.3.99.4 17alpha-methyltestosterone + acceptor
-
 Comamonas testosteroni 17alpha-methylandrost-1,4-diene-3-one + reduced acceptor
-
 ?
1.3.99.4 4-androstene-3,17-dione + acceptor
-
 Comamonas testosteroni 1,4-androstene-3,17-dione + reduced acceptor
-
 ir
1.3.99.4 4-pregnene-3,20-dione + acceptor
-
 Comamonas testosteroni 1,4-pregnene-3,20 dione + reduced acceptor
-
 ?
1.3.99.4 deoxycorticosterone + acceptor 4-pregnene-21-ol-3,20-dione Comamonas testosteroni 1,4-pregnene-21-ol-3,20-dione + reduced acceptor
-
 ?
1.3.99.4 additional information acceptor: phenazine methosulfate Comamonas testosteroni ?
-
 ?
1.3.99.4 testosterone + acceptor
-
 Comamonas testosteroni 17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor
-
 ?
1.3.99.5 1-androstene-3,17-dione + phenazine methosulfate
-
 Comamonas testosteroni 1,4-androstadiene-3,17-dione + reduced phenazine methosulfate
-
 ir
1.3.99.5 1-estrene-3,17-dione + phenazine methosulfate
-
 Comamonas testosteroni 1,4-estradiene-3,17-dione + reduced phenazine methosulfate
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.4 25
-
 assay at Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.4 9.5
-
-
 Comamonas testosteroni
1.3.99.5 9.5
-
-
 Comamonas testosteroni