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Literature summary extracted from

  • Aries, V.C.; Goddard, P.; Hill, M.J.
    Degradation of steroids by intestinal bacteria. III. 3-Oxo-5beta-steroid DELTA1-dehydrogenase and 3-oxo-5beta-steroid DELTA4-dehydrogenase (1971), Biochim. Biophys. Acta, 248, 482-488.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.6 Ca2+ 10 mM Clostridium paraputrificum
1.3.99.6 Cu2+ 10 mM Clostridium paraputrificum
1.3.99.6 formaldehyde 30 mM Clostridium paraputrificum
1.3.99.6 iodoacetate 50 mM, partial Clostridium paraputrificum
1.3.99.6 Merthiolate 1.0 mM Clostridium paraputrificum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.6 additional information Clostridium paraputrificum inducible by 3-oxo-5beta-cholan-24-oic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Clostridium paraputrificum
-
 3-oxo-5beta-steroid DELTA1-dehydrogenase is active only on 5-beta-steroids
-
1.3.99.6 Clostridium paraputrificum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 3-oxo-5beta-cholan-24-oic acid + acceptor
-
 Clostridium paraputrificum ? + reduced acceptor
-
 ?
1.3.99.4 5beta-androstan-3,17-dione + acceptor
-
 Clostridium paraputrificum 5beta-androst-1-en-3,17-dione + reduced acceptor
-
 ?
1.3.99.4 additional information acceptor: phenazine methosulfate Clostridium paraputrificum ?
-
 ?
1.3.99.4 additional information acceptor: O2 Clostridium paraputrificum ?
-
 ?
1.3.99.4 additional information 3-oxo-5beta-steroid DELTA1-dehydrogenase is active only on 5beta-steroids Clostridium paraputrificum ?
-
 ?
1.3.99.6 17beta-hydroxy-5beta-androstan-3-one + menaphthone acceptor: phenazine methosulfate Clostridium paraputrificum testosterone + reduced acceptor
-
 ?
1.3.99.6 3,12-dioxo-5beta-cholan-24-oic acid + menaphthone
-
 Clostridium paraputrificum 3,12-dioxo-4-cholen-24-oic acid + reduced menaphthone
-
 ?
1.3.99.6 3,12-dioxo-5beta-cholan-24-oic acid + phenazine methosulfate
-
 Clostridium paraputrificum 3,12-dioxo-4-cholen-24-oic acid + reduced phenazine methosulphate
-
 ?
1.3.99.6 3,12-dioxo-5beta-cholan-24-oic acid methyl ester + menaphthone
-
 Clostridium paraputrificum 3,12-dioxo-4-cholen-24 oic acid methyl ester + reduced menaphthone
-
 ?
1.3.99.6 3,12-dioxo-5beta-cholan-24-oic acid methyl ester + phenazine methosulfate
-
 Clostridium paraputrificum 3,12-dioxo-4-cholen-24 oic acid methyl ester + reduced phenazine methosulfate
-
 ?
1.3.99.6 3-oxo-5beta-24-cholan-oic acid + phenazine methosulfate
-
 Clostridium paraputrificum 3-oxo-4-cholen-24-oic acid + reduced phenazine methosulfate
-
 ?
1.3.99.6 3-oxo-5beta-cholan-24-oic acid + menaphthone
-
 Clostridium paraputrificum 3-oxo-4-cholen-24-oic acid + reduced menaphthone
-
 ?
1.3.99.6 5beta-androstan-3,17-dione + acceptor acceptor: phenazine methosulfate Clostridium paraputrificum 4-androsten-3,17-dione + reduced acceptor
-
 ?
1.3.99.6 5beta-pregnan-3,20-dione + menaphthone
-
 Clostridium paraputrificum 4-pregnen-3,20-dione + reduced menaphthone
-
 ?
1.3.99.6 5beta-pregnan-3,20-dione + phenazine methosulfate
-
 Clostridium paraputrificum 4-pregnen-3,20-dione + reduced menazine methosulfate
-
 ?
1.3.99.6 additional information no activity with: NAD+, NADP+, FMN and FAD Clostridium paraputrificum ?
-
 ?
1.3.99.6 additional information inducible by 3-oxo-5beta-cholan-24-oic acid Clostridium paraputrificum ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.99.6 58
-
 half-life: 10 min Clostridium paraputrificum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.4 8 9 3-oxo-5beta-steroid DELTA1-dehydrogenase is active only on 5beta-steroids Clostridium paraputrificum
1.3.99.6 7 8
-
 Clostridium paraputrificum