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Literature summary extracted from

  • Nandy, A.; Kieweg, V.; Kraeutle, F.G.; Vock, P.; Kuechler, B.; Bross, P.; Kim, J.J.P.; Rasched, I.; Ghisla, S.
    Medium-long-chain chimeric human acyl-CoA dehydrogenase: medium-chain enzyme with the active center base arrangement of long-chain acyl-CoA dehydrogenase (1996), Biochemistry, 35, 12402-12411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.8 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.7 T255E same activity with octanoyl-CoA as wild-type, activity/chain length profile is, however, narrower Homo sapiens
1.3.8.7 T255E/E376G chimeric medium-long chain acyl-CoA dehydrogenase, has 20% of the activity of medium-chain acyl-CoA dehydrogenase and 25% that of long-chain acyl-CoA dehydrogenase activity with its best substrates octanoyl-CoA and dodecanoyl-CoA respectively, activity maximum is shifted to C12 and C14-CoA, enzyme shows an enhanced rate of reoxidation with oxygen Homo sapiens
1.3.8.7 T255E/E376T shifts the chain length activity profile to higher values, drastic reduction of maximal velocity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.8 2-octynoyl-CoA inactivation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.7 0.001
-
 octanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 0.0013
-
 hexadecanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 0.0014
-
 hexanoyl-CoA T255E mutant enzyme Homo sapiens
1.3.8.7 0.0016
-
 hexadecanoyl-CoA
-
 Homo sapiens
1.3.8.7 0.0023
-
 tetradecanoyl-CoA
-
 Homo sapiens
1.3.8.7 0.0025
-
 decanoyl-CoA
-
 Homo sapiens
1.3.8.7 0.0025
-
 dodecanoyl-CoA
-
 Homo sapiens
1.3.8.7 0.0034
-
 octanoyl-CoA
-
 Homo sapiens
1.3.8.7 0.0042
-
 decanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 0.0055
-
 tetradecanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 0.0066
-
 dodecanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 0.008
-
 octanoyl-CoA T255E mutant enzyme Homo sapiens
1.3.8.7 0.01
-
 decanoyl-CoA T255E mutant enzyme Homo sapiens
1.3.8.7 0.015
-
 hexanoyl-CoA
-
 Homo sapiens
1.3.8.7 0.05
-
 decanoyl-CoA T255E/E376T double mutant enzyme Homo sapiens
1.3.8.7 0.05
-
 dodecanoyl-CoA T255E/E376T double mutant enzyme Homo sapiens
1.3.8.7 0.07
-
 butanoyl-CoA T255E mutant enzyme Homo sapiens
1.3.8.7 0.175
-
 butanoyl-CoA
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.7 Homo sapiens
-
-
-
1.3.8.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.7 recombinant wild-type, T255E, T255E/E367G and T255E/E367T mutant enzymes Homo sapiens
1.3.8.8
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.8.7 a medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein the catalytic base responsible for the alpha-proton abstraction is E376 in medium-chain acyl-CoA dehydrogenase, while that in long-chain acyl-CoA dehydrogenase is E255 Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.7 butanoyl-CoA + acceptor
-
 Homo sapiens crotonyl-CoA + reduced acceptor
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.7 0.167
-
 octanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 0.233
-
 decanoyl-CoA T255E/E376T double mutant enzyme Homo sapiens
1.3.8.7 0.333
-
 decanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 0.617
-
 hexadecanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 2.33
-
 hexadecanoyl-CoA
-
 Homo sapiens
1.3.8.7 2.67
-
 tetradecanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 2.83
-
 hexanoyl-CoA T255E mutant enzyme Homo sapiens
1.3.8.7 3.33
-
 dodecanoyl-CoA T255E/E376G double mutant enzyme Homo sapiens
1.3.8.7 4.67
-
 tetradecanoyl-CoA
-
 Homo sapiens
1.3.8.7 8.33
-
 butanoyl-CoA wild-type and T255E mutant enzyme Homo sapiens
1.3.8.7 9.17
-
 dodecanoyl-CoA
-
 Homo sapiens
1.3.8.7 9.67
-
 decanoyl-CoA
-
 Homo sapiens
1.3.8.7 11.3
-
 decanoyl-CoA T255E mutant enzyme Homo sapiens
1.3.8.7 13.2
-
 octanoyl-CoA T255E mutant enzyme Homo sapiens
1.3.8.7 16.3
-
 octanoyl-CoA
-
 Homo sapiens
1.3.8.7 16.8
-
 hexanoyl-CoA
-
 Homo sapiens