Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hall, C.L.; Heijkenskjöld, L.; Bartfai, T.; Ernster, L.; Kamin, H.
    Acyl coenzyme A dehydrogenases and electron-transferring flavoprotein from beef hart mitochondria (1976), Arch. Biochem. Biophys., 177, 402-414.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.8 palmitoyl-CoA inhibition by micelle formation at concentrations up to 0.004 mM, reversed by fatty acid free bovine serum albumin Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.7 0.0003 0.001 electron transferring flavoprotein
-
 Bos taurus
1.3.8.7 0.001
-
 hexadecanoyl-CoA in the presence of bovine serum albumin Bos taurus
1.3.8.8 0.001
-
 palmitoyl-CoA
-
 Bos taurus
1.3.8.8 0.001
-
 palmitoyl-CoA in the presence of fatty acid free bovine serum albumin Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.8 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.7 Bos taurus
-
-
-
1.3.8.8 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.7
-
 Bos taurus
1.3.8.8 40-75% ammonium sulfate precipitation, G-200 electrophoresis Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.7 heart
-
 Bos taurus
-
1.3.8.8 heart
-
 Bos taurus
-
1.3.8.8 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.7 0.076
-
 substrate butanoyl-CoA Bos taurus
1.3.8.7 0.168
-
 substrate hexadecanoyl-CoA Bos taurus
1.3.8.7 0.272
-
 substrate octanoyl-CoA Bos taurus
1.3.8.8 0.076
-
 purified enzyme, butyryl-CoA Bos taurus
1.3.8.8 0.19
-
 purified enzyme, palmitoyl-CoA, 0.12 nmol/ml ETF Bos taurus
1.3.8.8 0.259
-
 purified enzyme, palmitoyl-CoA, 0.24 nmol/ml ETF Bos taurus
1.3.8.8 0.272
-
 purified enzyme, octanoyl-CoA Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.7 hexadecanoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
-
 Bos taurus 2-trans-hexadecenoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 octanoyl-CoA + electron transferring flavoprotein
-
 Bos taurus 2-octenoyl-CoA + reduced electron transferring flavoprotein
-
 ?
1.3.8.8 butyryl-CoA + electron-transfer flavoprotein
-
 Bos taurus 2-butenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 octanoyl-CoA + electron-transfer flavoprotein
-
 Bos taurus 2-octenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 palmitoyl-CoA + electron-transfer flavoprotein
-
 Bos taurus 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.8 tetramer 4 * 38000-45200, SDS-PAGE Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.7 1.17 2 electron transferring flavoprotein
-
 Bos taurus
1.3.8.8 1.67
-
 palmitoyl-CoA saturating ETF Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.8 FAD
-
 Bos taurus