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Literature summary extracted from

  • Van Berkel, W.J.H.; Van den Berg, W.A.M.; Muller, F.
    Large-scale preparation and reconstitution of apo-flavoproteins with special reference to butyryl-CoA dehydrogenase from Megasphaera elsdenii. Hydrophobic-interaction chromatography [published erratum appears in Eur J Biochem 1989 Apr 1;180(3):603] (1988), Eur. J. Biochem., 178, 197-207.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.1 acetoacetyl-CoA 0.5 mM, 60% inhibition, apo-enzyme reconstituted with FAD Megasphaera elsdenii

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Megasphaera elsdenii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.1 preparation of apoprotein Megasphaera elsdenii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.3.8.1 reconstitution of holoenzyme after removal of FAD Megasphaera elsdenii

Storage Stability

EC Number Storage Stability Organism
1.3.8.1 -18°C, 50% ethylene glycol, 6 months, 80% activity after reconstitution with FAD Megasphaera elsdenii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.8.1 6
-
 most stable Megasphaera elsdenii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.8.1 0.00012
-
 acetoacetyl-CoA apo-enzyme reconstituted with FAD Megasphaera elsdenii