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Literature summary extracted from

  • Hines, V.; Johnston, M.
    Analysis of the kinetic mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase (1989), Biochemistry, 28, 1222-1226.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 Barbituric acid
-
 Bos taurus
1.3.5.2 Orotate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.009
-
 dihydroorotate
-
 Bos taurus
1.3.5.2 0.0092
-
 S-dihydroorotate
-
 Bos taurus
1.3.5.2 0.014
-
 coenzyme Q6
-
 Bos taurus
1.3.5.2 0.0142
-
 coenzyme Q6
-
 Bos taurus
1.3.5.2 0.0154
-
 methyl dihydroorotate
-
 Bos taurus
1.3.5.2 0.1542
-
 methyl dihydroorotate
-
 Bos taurus
1.3.5.2 0.184
-
 menadione
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.5.2 Iron iron-sulfur center Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Bos taurus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.5.2 (S)-dihydroorotate + a quinone = orotate + a quinol ping-pong mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.2 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + menadione
-
 Bos taurus orotate + menadiol
-
 r
1.3.5.2 (S)-dihydroorotate + ubiquinone
-
 Bos taurus orotate + ubiquinol
-
 ?
1.3.5.2 dihydroorotate + acceptor acceptor: coenzyme Q6 Bos taurus orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor acceptor: menadione Bos taurus orotate + reduced acceptor
-
 ?
1.3.5.2 L-dihydroorotate + ubiquinone-6
-
 Bos taurus orotate + reduced ubiquinone-6
-
 ?
1.3.5.2 methyl-dihydroorotate + acceptor
-
 Bos taurus methyl-orotate + reduced acceptor
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.2 18.3
-
 menadione
-
 Bos taurus
1.3.5.2 44.2
-
 coenzyme Q6
-
 Bos taurus
1.3.5.2 45.4
-
 methyl-dihydroorotate
-
 Bos taurus
1.3.5.2 50.4
-
 dihydroorotate
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.2 FMN
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.2 0.005
-
 Orotic acid competitive to dihydroorotate Bos taurus
1.3.5.2 0.092
-
 Barbiturate
-
 Bos taurus