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Literature summary extracted from

  • Maeshima, M.; Hattori, T.; Asahi, T.
    Purification of complexes II and IV from plant mitochondria (1987), Methods Enzymol., 148, 491-501.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.3.5.1 extremely labile Ipomoea batatas
7.1.1.9 inactivation by centrifugation of pure complex Ipomoea batatas

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.9 KCl 50 mM, 50% inhibition Ipomoea batatas
7.1.1.9 phosphate more than 15 mM Ipomoea batatas
7.1.1.9 Triton X-100 0.3%, 50% inhibition Ipomoea batatas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.29
-
 succinate
-
 Ipomoea batatas

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 mitochondrion
-
 Ipomoea batatas 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.1 26000
-
 1 * 65000 + 1 * 26000, large subunit is a flavoprotein, small subunit is an iron-sulfur protein Ipomoea batatas
1.3.5.1 65000
-
 1 * 65000 + 1 * 26000, large subunit is a flavoprotein, small subunit is an iron-sulfur protein Ipomoea batatas
1.3.5.1 110000
-
 PAGE Ipomoea batatas
7.1.1.9 5700
-
 I, II, III, IV, V, x * 39000 + x * 33500 + x * 26000 + x * 20000 + x * 5700, probably 2 more small subunits, SDS-PAGE Ipomoea batatas
7.1.1.9 20000
-
 I, II, III, IV, V, x * 39000 + x * 33500 + x * 26000 + x * 20000 + x * 5700, probably 2 more small subunits, SDS-PAGE Ipomoea batatas
7.1.1.9 26000
-
 I, II, III, IV, V, x * 39000 + x * 33500 + x * 26000 + x * 20000 + x * 5700, probably 2 more small subunits, SDS-PAGE Ipomoea batatas
7.1.1.9 33500
-
 I, II, III, IV, V, x * 39000 + x * 33500 + x * 26000 + x * 20000 + x * 5700, probably 2 more small subunits, SDS-PAGE Ipomoea batatas
7.1.1.9 39000
-
 I, II, III, IV, V, x * 39000 + x * 33500 + x * 26000 + x * 20000 + x * 5700, probably 2 more small subunits, SDS-PAGE Ipomoea batatas

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Ipomoea batatas
-
 sweet potato, succinate dehydrogenase
-
7.1.1.9 Ipomoea batatas
-
 sweet potato
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
7.1.1.9 phospholipoprotein
-
 Ipomoea batatas

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.1 two forms, differing in protein charges Ipomoea batatas
7.1.1.9 DEAE-cellulose, ammonium sulfate Ipomoea batatas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.1 parenchyma
-
 Ipomoea batatas
-
7.1.1.9 root
-
 Ipomoea batatas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.9 147
-
 cytochrome c Ipomoea batatas

Storage Stability

EC Number Storage Stability Organism
1.3.5.1 -80°C, anaerobic conditions Ipomoea batatas
7.1.1.9 -70 stable Ipomoea batatas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 succinate + phenazine methosulfate
-
 Ipomoea batatas fumarate + reduced phenazine methosulfate
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.5.1 dimer 1 * 65000 + 1 * 26000, large subunit is a flavoprotein, small subunit is an iron-sulfur protein Ipomoea batatas
7.1.1.9 ? I, II, III, IV, V, x * 39000 + x * 33500 + x * 26000 + x * 20000 + x * 5700, probably 2 more small subunits, SDS-PAGE Ipomoea batatas

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.1.1.9 4
-
 12 h: inactivation Ipomoea batatas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.1 7.8 8
-
 Ipomoea batatas