BRENDA - Enzyme Database

Resolution of complex II and isolation of succinate dehydrogenase

Hatefi, Y.; Methods Enzymol. 53, 27-35 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
fumarate
-
Mammalia
1.3.5.1
Maleate
-
Mammalia
1.3.5.1
malonate
-
Mammalia
1.3.5.1
Methylene succinate
-
Mammalia
1.3.5.1
oxaloacetate
-
Mammalia
1.3.5.1
thiol reagents
-
Mammalia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.48
-
phenazine methosulfate
-
Mammalia
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
mitochondrion
cytoplasmic side of inner membrane
Mammalia
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Fe-S-clusters
-
Mammalia
1.3.5.1
Iron
-
Mammalia
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.1
7000
-
1 * 7000 + 1* 27000, SDS-PAGE
Mammalia
1.3.5.1
97000
105000
sedimentation
Mammalia
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Mammalia
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.5.1
from complex II, i.e. EC 1.3.5.1
Mammalia
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.3.5.1
67
78
-
Mammalia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + electron acceptor
main reaction for succinate dehydrogenase, acceptor: phenazine methosulfate (oxidized)
391153
Mammalia
fumarate + reduced acceptor
-
-
-
?
1.3.5.1
succinate + electron acceptor
acceptor: ferricyanide
391153
Mammalia
fumarate + reduced acceptor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.5.1
dimer
1 * 7000 + 1* 27000, SDS-PAGE
Mammalia
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.5.1
167
217
succinate
-
Mammalia
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
FAD
covalently linked to protein histidyl residue
Mammalia
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.0015
-
oxaloacetate
-
Mammalia
1.3.5.1
0.018
-
malonate
-
Mammalia
1.3.5.1
1.3
-
fumarate
-
Mammalia
1.3.5.1
1.8
-
Methylene succinate
-
Mammalia
1.3.5.1
6.2
-
Maleate
-
Mammalia
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
FAD
covalently linked to protein histidyl residue
Mammalia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
fumarate
-
Mammalia
1.3.5.1
Maleate
-
Mammalia
1.3.5.1
malonate
-
Mammalia
1.3.5.1
Methylene succinate
-
Mammalia
1.3.5.1
oxaloacetate
-
Mammalia
1.3.5.1
thiol reagents
-
Mammalia
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.0015
-
oxaloacetate
-
Mammalia
1.3.5.1
0.018
-
malonate
-
Mammalia
1.3.5.1
1.3
-
fumarate
-
Mammalia
1.3.5.1
1.8
-
Methylene succinate
-
Mammalia
1.3.5.1
6.2
-
Maleate
-
Mammalia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.48
-
phenazine methosulfate
-
Mammalia
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
mitochondrion
cytoplasmic side of inner membrane
Mammalia
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Fe-S-clusters
-
Mammalia
1.3.5.1
Iron
-
Mammalia
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.1
7000
-
1 * 7000 + 1* 27000, SDS-PAGE
Mammalia
1.3.5.1
97000
105000
sedimentation
Mammalia
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.1
from complex II, i.e. EC 1.3.5.1
Mammalia
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.3.5.1
67
78
-
Mammalia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + electron acceptor
main reaction for succinate dehydrogenase, acceptor: phenazine methosulfate (oxidized)
391153
Mammalia
fumarate + reduced acceptor
-
-
-
?
1.3.5.1
succinate + electron acceptor
acceptor: ferricyanide
391153
Mammalia
fumarate + reduced acceptor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.5.1
dimer
1 * 7000 + 1* 27000, SDS-PAGE
Mammalia
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.5.1
167
217
succinate
-
Mammalia