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Literature summary extracted from

  • Holcenberg, J.S.; Tsai, L.
    Nicotinic acid metabolism. IV. Ferredoxin-dependent reduction of 6-hydroxynicotinic acid to 6-oxo-1,4,5,6-tetrahydronicotinic acid (1969), J. Biol. Chem., 244, 1204-1211.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.7.1 arsenate crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity Clostridium sp.
1.3.7.1 phosphate crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity Clostridium sp.
1.3.7.1 pyruvate crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity Clostridium sp.

General Stability

EC Number General Stability Organism
1.3.7.1 cobinamide cyanide, 0.01 mM, accelerates loss of activity in air Clostridium sp.
1.3.7.1 ethylene glycol high concentrations stabilize Clostridium sp.
1.3.7.1 glycerol, concentrations above 30% stabilize Clostridium sp.
1.3.7.1 Na2S * 9 H2O stabilizes, maximal effect at 0.03% Clostridium sp.
1.3.7.1 stable to freezing and thawing Clostridium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.7.1 0.016
-
 reduced ferredoxin
-
 Clostridium sp.
1.3.7.1 0.3
-
 reduced methyl viologen
-
 Clostridium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.1 Clostridium sp.
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.3.7.1 air, 5°C, 24 h, 50% loss of activity Clostridium sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.7.1 partial Clostridium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.7.1 1.9
-
-
 Clostridium sp.

Storage Stability

EC Number Storage Stability Organism
1.3.7.1 several months in liquid nitrogen and up to 50 h with argon Clostridium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.1 1,4,5,6-tetrahydro-6-oxonicotinate + oxidized ferredoxin oxidized methyl viologen is also substrate Clostridium sp. 6-hydroxynicotinate + reduced ferredoxin
-
 r
1.3.7.1 6-hydroxynicotinate + reduced ferredoxin
-
 Clostridium sp. 6-oxo-1,4,5,6-tetrahydronicotinate + oxidized ferredoxin
-
 r
1.3.7.1 6-hydroxynicotinate + reduced methyl viologen
-
 Clostridium sp. 6-oxo-1,4,5,6-tetrahydronicotinate + oxidized methyl viologen
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.7.1 25
-
 assay at Clostridium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.7.1 5
-
 stable in liquid nitrogen, unstable in air Clostridium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.7.1 6.5
-
-
 Clostridium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.7.1 5.5 8.2 pH 5.5: about 60% of activity maximum, pH 8.2: about 20% of activity maximum, rapid decline in activity below pH 5.6 may be partly due to destruction of ferredoxin in acid Clostridium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.7.1 additional information
-
-
 Clostridium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.1 CoA crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity Clostridium sp.
1.3.7.1 Ferredoxin reduced or oxidized, as electron carrier Clostridium sp.