BRENDA - Enzyme Database

Comparison of catalytic activity and inhibitors of quinone reactions of succinate dehydrogenase (Succinate-ubiquinone oxidoreductase) and fumarate reductase (Menaquinol-fumarate oxidoreductase) from Escherichia coli

Maklashina, E.; Cecchini, G.; Arch. Biochem. Biophys. 369, 223-232 (1999)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
2-(n-heptyl)-4-hydroxy-quinoline N-oxide
potent inhibitor of fumarate reductase, not inhibitory for succinate dehydrogenase
Escherichia coli
1.3.5.1
2-alkyl-4,6-dinitrophenol-17
-
Escherichia coli
1.3.5.1
2-alkyl-4,6-dinitrophenol-20
-
Escherichia coli
1.3.5.1
2-alkyl-4,6-dinitrophenols
derivatives of 2-alkyl-4,6-dinitrophenols, competitive inhibitors of both succinate dehydrogenase and fumarate reductase
Escherichia coli
1.3.5.1
carboxin
inhibits the succinate dehydrogenase in the forward and reverse reaction
Escherichia coli
1.3.5.1
malonate
inhibits both succinate dehydrogenase and fumarate reductase
Escherichia coli
1.3.5.1
menaquinone-1
competitive inhibitor of the succinate oxidation reaction of succinate dehydrogenase
Escherichia coli
1.3.5.1
Pentachlorophenol
competitive inhibitor of both succinate dehydrogenase and fumarate reductase
Escherichia coli
1.3.5.1
ubiquinol-2
competitive inhibitor of the fumarate reduction reaction of fumarate reductase
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
additional information
-
additional information
succinate-quinone and quinol-fumarate reductase reaction of succinate dehydrogenase and fumarate reductase
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
membrane
-
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.1
fumarate + menaquinol
Escherichia coli
fumarate reductase acts as part of an anaerobic respiratory chain
succinate + menaquinone
-
-
r
1.3.5.1
additional information
Escherichia coli
succinate dehydrogenase is a component of the respiratory chain and operates as a compulsory member of the Krebs cycle in mammals
?
-
-
-
1.3.5.1
succinate + ubiquinone
Escherichia coli
-
fumarate + ubiquinol
-
Escherichia coli
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Escherichia coli
-
fumarate reductase; succinate dehydrogenase
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.5.1
of both succinate dehydrogenase and fumarate reductase using solubilization with Thesit and DEAE fast-flow chromatography
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
fumarate + menaquinol
fumarate reductase acts as part of an anaerobic respiratory chain
391137
Escherichia coli
succinate + menaquinone
-
-
-
r
1.3.5.1
fumarate + quinol
-
391137
Escherichia coli
succinate + ubiquinone
-
391137
Escherichia coli
r
1.3.5.1
additional information
succinate dehydrogenase is a component of the respiratory chain and operates as a compulsory member of the Krebs cycle in mammals
391137
Escherichia coli
?
-
-
-
-
1.3.5.1
succinate + ubiquinone
-
391137
Escherichia coli
fumarate + ubiquinol
-
391137
Escherichia coli
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.5.1
additional information
-
additional information
succinate-quinone and quinol-fumarate reductase reaction of succinate dehydrogenase and fumarate reductase
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
7.4
7.8
for both succinate dehydrogenase and fumarate reductase
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.000075
-
2-(n-heptyl)-4-hydroxy-quinoline N-oxide
fumarate reductase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.0002
-
2-(n-heptyl)-4-hydroxy-quinoline N-oxide
fumarate reductase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.0013
-
2-alkyl-4,6-dinitrophenol-17
fumarate reductase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.0015
-
2-alkyl-4,6-dinitrophenol-20
fumarate reductase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.002
-
2-alkyl-4,6-dinitrophenol-17
fumarate reductase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.002
-
2-alkyl-4,6-dinitrophenol-20
fumarate reductase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.012
-
menaquinone-1
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.013
-
Pentachlorophenol
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.015
-
ubiquinol-2
fumarate reductase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.017
-
2-alkyl-4,6-dinitrophenol-17
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.017
-
Pentachlorophenol
succinate dehydrogenase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.019
-
2-alkyl-4,6-dinitrophenol-20
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.023
-
Pentachlorophenol
fumarate reductase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.03
-
carboxin
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.035
-
carboxin
succinate dehydrogenase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.037
-
Pentachlorophenol
fumarate reductase, fumarate reduction reaction
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
2-(n-heptyl)-4-hydroxy-quinoline N-oxide
potent inhibitor of fumarate reductase, not inhibitory for succinate dehydrogenase
Escherichia coli
1.3.5.1
2-alkyl-4,6-dinitrophenol-17
-
Escherichia coli
1.3.5.1
2-alkyl-4,6-dinitrophenol-20
-
Escherichia coli
1.3.5.1
2-alkyl-4,6-dinitrophenols
derivatives of 2-alkyl-4,6-dinitrophenols, competitive inhibitors of both succinate dehydrogenase and fumarate reductase
Escherichia coli
1.3.5.1
carboxin
inhibits the succinate dehydrogenase in the forward and reverse reaction
Escherichia coli
1.3.5.1
malonate
inhibits both succinate dehydrogenase and fumarate reductase
Escherichia coli
1.3.5.1
menaquinone-1
competitive inhibitor of the succinate oxidation reaction of succinate dehydrogenase
Escherichia coli
1.3.5.1
Pentachlorophenol
competitive inhibitor of both succinate dehydrogenase and fumarate reductase
Escherichia coli
1.3.5.1
ubiquinol-2
competitive inhibitor of the fumarate reduction reaction of fumarate reductase
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.000075
-
2-(n-heptyl)-4-hydroxy-quinoline N-oxide
fumarate reductase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.0002
-
2-(n-heptyl)-4-hydroxy-quinoline N-oxide
fumarate reductase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.0013
-
2-alkyl-4,6-dinitrophenol-17
fumarate reductase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.0015
-
2-alkyl-4,6-dinitrophenol-20
fumarate reductase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.002
-
2-alkyl-4,6-dinitrophenol-17
fumarate reductase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.002
-
2-alkyl-4,6-dinitrophenol-20
fumarate reductase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.012
-
menaquinone-1
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.013
-
Pentachlorophenol
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.015
-
ubiquinol-2
fumarate reductase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.017
-
2-alkyl-4,6-dinitrophenol-17
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.017
-
Pentachlorophenol
succinate dehydrogenase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.019
-
2-alkyl-4,6-dinitrophenol-20
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.023
-
Pentachlorophenol
fumarate reductase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.03
-
carboxin
succinate dehydrogenase, succinate oxidation reaction
Escherichia coli
1.3.5.1
0.035
-
carboxin
succinate dehydrogenase, fumarate reduction reaction
Escherichia coli
1.3.5.1
0.037
-
Pentachlorophenol
fumarate reductase, fumarate reduction reaction
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
additional information
-
additional information
succinate-quinone and quinol-fumarate reductase reaction of succinate dehydrogenase and fumarate reductase
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
membrane
-
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.1
fumarate + menaquinol
Escherichia coli
fumarate reductase acts as part of an anaerobic respiratory chain
succinate + menaquinone
-
-
r
1.3.5.1
additional information
Escherichia coli
succinate dehydrogenase is a component of the respiratory chain and operates as a compulsory member of the Krebs cycle in mammals
?
-
-
-
1.3.5.1
succinate + ubiquinone
Escherichia coli
-
fumarate + ubiquinol
-
Escherichia coli
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.1
of both succinate dehydrogenase and fumarate reductase using solubilization with Thesit and DEAE fast-flow chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
fumarate + menaquinol
fumarate reductase acts as part of an anaerobic respiratory chain
391137
Escherichia coli
succinate + menaquinone
-
-
-
r
1.3.5.1
fumarate + quinol
-
391137
Escherichia coli
succinate + ubiquinone
-
391137
Escherichia coli
r
1.3.5.1
additional information
succinate dehydrogenase is a component of the respiratory chain and operates as a compulsory member of the Krebs cycle in mammals
391137
Escherichia coli
?
-
-
-
-
1.3.5.1
succinate + ubiquinone
-
391137
Escherichia coli
fumarate + ubiquinol
-
391137
Escherichia coli
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.5.1
additional information
-
additional information
succinate-quinone and quinol-fumarate reductase reaction of succinate dehydrogenase and fumarate reductase
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
7.4
7.8
for both succinate dehydrogenase and fumarate reductase
Escherichia coli