BRENDA - Enzyme Database

Studies on the succinate dehydrogenating system. Isolation and properties of the mitochondrial succinate-ubiquinone reductase

Tushurashvili, P.R.; Gavrikova, E.V.; Ledenev, A.N.; Vinogradov, A.D.; Biochim. Biophys. Acta 809, 145-159 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
2-Thenoyltrifluoroacetone
-
Bos taurus
1.3.5.1
carboxin
Q2-mediated Wurster's blue reduction: 90% inhibition at 0.02 mM, Q0-mediated Wurster's blue reduction: 75% inhibition at 0.02 mM, Q1-mediated Wurster's blue reduction: 88% inhibition at 0.02 mM, Q6-mediated Wurster's blue reduction: 50% inhibition at 0.02 mM
Bos taurus
1.3.5.1
fumarate
-
Bos taurus
1.3.5.1
malonate
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.0005
-
ubiquinone-2
-
Bos taurus
1.3.5.1
0.02
-
succinate
-
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Fe
-
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Bos taurus
-
succinate dehydrogenase
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.5.1
using urea washing, Triton X-100 extraction, cholate-ammonium sulfate extraction, calcium phosphate gel and ammonium sulfate precipitation
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.5.1
heart
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.5.1
28
54
depending on assay temperature
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
1.3.5.1
liquid N2 or -20°C, pH 6.0, presence of 0.1% Triton X-100
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone
-
391123
Bos taurus
fumarate + 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinol
-
-
-
-
1.3.5.1
succinate + ubiquinone
-
391123
Bos taurus
fumarate + ubiquinol
-
391123
Bos taurus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.5.1
tetramer
heart, succinate dehydrogenase, SDS-PAGE
Bos taurus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.5.1
83.3
150
succinate
based on FAD content, dependency on assay temperature
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
6
-
-
Bos taurus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.3.5.1
5
8
inactivation below pH 5, stable for about 20 h without loss of activity at pH 6.0 in the presence of 0.1% Triton X-100
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
cytochrome b
-
Bos taurus
1.3.5.1
cytochrome b-560
-
Bos taurus
1.3.5.1
FAD
-
Bos taurus
1.3.5.1
additional information
the enzyme contains acid-labile sulfides
Bos taurus
1.3.5.1
ubiquinone
-
Bos taurus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.00025
-
malonate
-
Bos taurus
1.3.5.1
0.001
-
carboxin
-
Bos taurus
1.3.5.1
0.008
-
2-Thenoyltrifluoroacetone
-
Bos taurus
1.3.5.1
0.04
-
fumarate
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
cytochrome b
-
Bos taurus
1.3.5.1
cytochrome b-560
-
Bos taurus
1.3.5.1
FAD
-
Bos taurus
1.3.5.1
additional information
the enzyme contains acid-labile sulfides
Bos taurus
1.3.5.1
ubiquinone
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
2-Thenoyltrifluoroacetone
-
Bos taurus
1.3.5.1
carboxin
Q2-mediated Wurster's blue reduction: 90% inhibition at 0.02 mM, Q0-mediated Wurster's blue reduction: 75% inhibition at 0.02 mM, Q1-mediated Wurster's blue reduction: 88% inhibition at 0.02 mM, Q6-mediated Wurster's blue reduction: 50% inhibition at 0.02 mM
Bos taurus
1.3.5.1
fumarate
-
Bos taurus
1.3.5.1
malonate
-
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.00025
-
malonate
-
Bos taurus
1.3.5.1
0.001
-
carboxin
-
Bos taurus
1.3.5.1
0.008
-
2-Thenoyltrifluoroacetone
-
Bos taurus
1.3.5.1
0.04
-
fumarate
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.0005
-
ubiquinone-2
-
Bos taurus
1.3.5.1
0.02
-
succinate
-
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Fe
-
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.1
using urea washing, Triton X-100 extraction, cholate-ammonium sulfate extraction, calcium phosphate gel and ammonium sulfate precipitation
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.5.1
heart
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.5.1
28
54
depending on assay temperature
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.3.5.1
liquid N2 or -20°C, pH 6.0, presence of 0.1% Triton X-100
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone
-
391123
Bos taurus
fumarate + 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinol
-
-
-
-
1.3.5.1
succinate + ubiquinone
-
391123
Bos taurus
fumarate + ubiquinol
-
391123
Bos taurus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.5.1
tetramer
heart, succinate dehydrogenase, SDS-PAGE
Bos taurus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.5.1
83.3
150
succinate
based on FAD content, dependency on assay temperature
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
6
-
-
Bos taurus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.3.5.1
5
8
inactivation below pH 5, stable for about 20 h without loss of activity at pH 6.0 in the presence of 0.1% Triton X-100
Bos taurus