BRENDA - Enzyme Database

Succinate dehydrogenase. A partial purification from mung bean hypocotyls and soybean cotyledons

Burke, J.J.; Siedow, J.N.; Moreland, D.E.; Plant Physiol. 70, 1577-1581 (1982)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
malonate
-
Glycine max
1.3.5.1
malonate
-
Vigna radiata
1.3.5.1
oxaloacetate
-
Glycine max
1.3.5.1
oxaloacetate
-
Vigna radiata
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
mitochondrion
inner membrane
Glycine max
5739
-
1.3.5.1
mitochondrion
inner membrane
Vigna radiata
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Fe
-
Glycine max
1.3.5.1
Fe
-
Vigna radiata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.1
13000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
13000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
1.3.5.1
15000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
15000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
1.3.5.1
30000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
30000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
1.3.5.1
67000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
67000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Glycine max
-
soybean, succinate dehydrogenase
-
1.3.5.1
Vigna radiata
-
mung bean, succinate dehydrogenase
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.3.5.1
O2, 22% loss of activity during solublization period, only 3% loss if solubilization is performed under Ar
Glycine max
1.3.5.1
O2, 22% loss of activity during solublization period, only 3% loss if solubilization is performed under Ar
Vigna radiata
Purification (Commentary)
EC Number
Commentary
Organism
1.3.5.1
partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation
Glycine max
1.3.5.1
partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation
Vigna radiata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.5.1
cotyledon
-
Glycine max
-
1.3.5.1
hypocotyl
-
Vigna radiata
-
Storage Stability
EC Number
Storage Stability
Organism
1.3.5.1
4°C, N2-atmosphere
Glycine max
1.3.5.1
4°C, N2-atmosphere
Vigna radiata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + ubiquinone
-
391114
Glycine max
fumarate + ubiquinol
-
391114
Glycine max
?
1.3.5.1
succinate + ubiquinone
-
391114
Vigna radiata
fumarate + ubiquinol
-
391114
Vigna radiata
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.5.1
tetramer
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
tetramer
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.5.1
4
-
enzyme activity remains constant for up to 5 h
Glycine max
1.3.5.1
4
-
enzyme activity remains constant for up to 5 h
Vigna radiata
1.3.5.1
25
-
62% loss of activity within 1 h
Glycine max
1.3.5.1
25
-
62% loss of activity within 1 h
Vigna radiata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
7
7.5
-
Glycine max
1.3.5.1
7
7.5
-
Vigna radiata
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
cytochrome b
-
Glycine max
1.3.5.1
cytochrome b
-
Vigna radiata
1.3.5.1
cytochrome b-560
-
Glycine max
1.3.5.1
cytochrome b-560
-
Vigna radiata
1.3.5.1
additional information
the enzyme contains iron-sulfur centers
Glycine max
1.3.5.1
additional information
the enzyme contains iron-sulfur centers
Vigna radiata
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.00034
-
oxaloacetate
-
Vigna radiata
1.3.5.1
0.00115
-
oxaloacetate
-
Glycine max
1.3.5.1
0.0218
-
malonate
-
Vigna radiata
1.3.5.1
0.0246
-
malonate
-
Glycine max
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
cytochrome b
-
Glycine max
1.3.5.1
cytochrome b
-
Vigna radiata
1.3.5.1
cytochrome b-560
-
Glycine max
1.3.5.1
cytochrome b-560
-
Vigna radiata
1.3.5.1
additional information
the enzyme contains iron-sulfur centers
Glycine max
1.3.5.1
additional information
the enzyme contains iron-sulfur centers
Vigna radiata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
malonate
-
Glycine max
1.3.5.1
malonate
-
Vigna radiata
1.3.5.1
oxaloacetate
-
Glycine max
1.3.5.1
oxaloacetate
-
Vigna radiata
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.00034
-
oxaloacetate
-
Vigna radiata
1.3.5.1
0.00115
-
oxaloacetate
-
Glycine max
1.3.5.1
0.0218
-
malonate
-
Vigna radiata
1.3.5.1
0.0246
-
malonate
-
Glycine max
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
mitochondrion
inner membrane
Glycine max
5739
-
1.3.5.1
mitochondrion
inner membrane
Vigna radiata
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Fe
-
Glycine max
1.3.5.1
Fe
-
Vigna radiata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.1
13000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
13000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
1.3.5.1
15000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
15000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
1.3.5.1
30000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
30000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
1.3.5.1
67000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
67000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.3.5.1
O2, 22% loss of activity during solublization period, only 3% loss if solubilization is performed under Ar
Glycine max
1.3.5.1
O2, 22% loss of activity during solublization period, only 3% loss if solubilization is performed under Ar
Vigna radiata
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.1
partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation
Glycine max
1.3.5.1
partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation
Vigna radiata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.5.1
cotyledon
-
Glycine max
-
1.3.5.1
hypocotyl
-
Vigna radiata
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.3.5.1
4°C, N2-atmosphere
Glycine max
1.3.5.1
4°C, N2-atmosphere
Vigna radiata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + ubiquinone
-
391114
Glycine max
fumarate + ubiquinol
-
391114
Glycine max
?
1.3.5.1
succinate + ubiquinone
-
391114
Vigna radiata
fumarate + ubiquinol
-
391114
Vigna radiata
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.5.1
tetramer
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Glycine max
1.3.5.1
tetramer
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
Vigna radiata
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.5.1
4
-
enzyme activity remains constant for up to 5 h
Glycine max
1.3.5.1
4
-
enzyme activity remains constant for up to 5 h
Vigna radiata
1.3.5.1
25
-
62% loss of activity within 1 h
Glycine max
1.3.5.1
25
-
62% loss of activity within 1 h
Vigna radiata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
7
7.5
-
Glycine max
1.3.5.1
7
7.5
-
Vigna radiata