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Literature summary extracted from

  • Burke, J.J.; Siedow, J.N.; Moreland, D.E.
    Succinate dehydrogenase. A partial purification from mung bean hypocotyls and soybean cotyledons (1982), Plant Physiol., 70, 1577-1581.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 malonate
-
 Glycine max
1.3.5.1 malonate
-
 Vigna radiata
1.3.5.1 oxaloacetate
-
 Glycine max
1.3.5.1 oxaloacetate
-
 Vigna radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 mitochondrion inner membrane Glycine max 5739
-
1.3.5.1 mitochondrion inner membrane Vigna radiata 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.5.1 Fe
-
 Glycine max
1.3.5.1 Fe
-
 Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.1 13000
-
 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Glycine max
1.3.5.1 13000
-
 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Vigna radiata
1.3.5.1 15000
-
 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Glycine max
1.3.5.1 15000
-
 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Vigna radiata
1.3.5.1 30000
-
 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Glycine max
1.3.5.1 30000
-
 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Vigna radiata
1.3.5.1 67000
-
 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Glycine max
1.3.5.1 67000
-
 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Vigna radiata

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Glycine max
-
 soybean, succinate dehydrogenase
-
1.3.5.1 Vigna radiata
-
 mung bean, succinate dehydrogenase
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.3.5.1 O2, 22% loss of activity during solublization period, only 3% loss if solubilization is performed under Ar Glycine max
1.3.5.1 O2, 22% loss of activity during solublization period, only 3% loss if solubilization is performed under Ar Vigna radiata

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.1 partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation Glycine max
1.3.5.1 partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation Vigna radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.1 cotyledon
-
 Glycine max
-
1.3.5.1 hypocotyl
-
 Vigna radiata
-

Storage Stability

EC Number Storage Stability Organism
1.3.5.1 4°C, N2-atmosphere Glycine max
1.3.5.1 4°C, N2-atmosphere Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 succinate + ubiquinone
-
 Glycine max fumarate + ubiquinol
-
 ?
1.3.5.1 succinate + ubiquinone
-
 Vigna radiata fumarate + ubiquinol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.1 tetramer 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Glycine max
1.3.5.1 tetramer 1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE Vigna radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.5.1 4
-
 enzyme activity remains constant for up to 5 h Glycine max
1.3.5.1 4
-
 enzyme activity remains constant for up to 5 h Vigna radiata
1.3.5.1 25
-
 62% loss of activity within 1 h Glycine max
1.3.5.1 25
-
 62% loss of activity within 1 h Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.1 7 7.5
-
 Glycine max
1.3.5.1 7 7.5
-
 Vigna radiata

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 cytochrome b
-
 Glycine max
1.3.5.1 cytochrome b
-
 Vigna radiata
1.3.5.1 cytochrome b-560
-
 Glycine max
1.3.5.1 cytochrome b-560
-
 Vigna radiata
1.3.5.1 additional information the enzyme contains iron-sulfur centers Glycine max
1.3.5.1 additional information the enzyme contains iron-sulfur centers Vigna radiata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.1 0.00034
-
 oxaloacetate
-
 Vigna radiata
1.3.5.1 0.00115
-
 oxaloacetate
-
 Glycine max
1.3.5.1 0.0218
-
 malonate
-
 Vigna radiata
1.3.5.1 0.0246
-
 malonate
-
 Glycine max