BRENDA - Enzyme Database

Kinetic characterization of succinate dehydrogenase (succinate: ubiquinone oxidoreductase) from Paracoccus denitrificans

Dadak, V.; Mala, G.; Kucera, I.; Biologia (Bratisl. ) 43, 1077-1085 (1988)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.5.1
5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole
slightly activates
Paracoccus denitrificans
1.3.5.1
antimycin A
activates
Paracoccus denitrificans
1.3.5.1
KCN
activation at 6.7 mM up to 10 mM when both alternative pathways to oxygen are inhibited
Paracoccus denitrificans
1.3.5.1
malonate
activation
Paracoccus denitrificans
1.3.5.1
Mucidin
activates
Paracoccus denitrificans
1.3.5.1
succinate
activation
Paracoccus denitrificans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
5-(n-Undecyl)-6-hydroxy-4,7-oxobenzothiazole
UHDBT
Paracoccus denitrificans
1.3.5.1
CN-
not inhibitory: when mixed with succinate or alone
Paracoccus denitrificans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.27
-
succinate
for both membrane fragments and mitochondrial enzyme
Paracoccus denitrificans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
membrane
-
Paracoccus denitrificans
16020
-
1.3.5.1
mitochondrion
-
Paracoccus denitrificans
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Paracoccus denitrificans
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.3.5.1
0.01
-
measured by the rate of dichloroindophenol reduction
Paracoccus denitrificans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + ubiquinone
-
391087
Paracoccus denitrificans
fumarate + ubiquinol
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
7
7.2
phosphate buffer
Paracoccus denitrificans
1.3.5.1
7.3
-
Tris-acetate buffer
Paracoccus denitrificans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.5.1
6
7.5
slow decline of activity at acidic pH, fast decline in alkaline range
Paracoccus denitrificans
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.3.5.1
7.5
-
enzyme activity falls abruptly
Paracoccus denitrificans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.5.1
5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole
slightly activates
Paracoccus denitrificans
1.3.5.1
antimycin A
activates
Paracoccus denitrificans
1.3.5.1
KCN
activation at 6.7 mM up to 10 mM when both alternative pathways to oxygen are inhibited
Paracoccus denitrificans
1.3.5.1
malonate
activation
Paracoccus denitrificans
1.3.5.1
Mucidin
activates
Paracoccus denitrificans
1.3.5.1
succinate
activation
Paracoccus denitrificans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
5-(n-Undecyl)-6-hydroxy-4,7-oxobenzothiazole
UHDBT
Paracoccus denitrificans
1.3.5.1
CN-
not inhibitory: when mixed with succinate or alone
Paracoccus denitrificans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.27
-
succinate
for both membrane fragments and mitochondrial enzyme
Paracoccus denitrificans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
membrane
-
Paracoccus denitrificans
16020
-
1.3.5.1
mitochondrion
-
Paracoccus denitrificans
5739
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.3.5.1
0.01
-
measured by the rate of dichloroindophenol reduction
Paracoccus denitrificans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + ubiquinone
-
391087
Paracoccus denitrificans
fumarate + ubiquinol
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
7
7.2
phosphate buffer
Paracoccus denitrificans
1.3.5.1
7.3
-
Tris-acetate buffer
Paracoccus denitrificans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.5.1
6
7.5
slow decline of activity at acidic pH, fast decline in alkaline range
Paracoccus denitrificans
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.3.5.1
7.5
-
enzyme activity falls abruptly
Paracoccus denitrificans