BRENDA - Enzyme Database

The enzymes and the enzyme complexes of the mitochondrial oxidative phosphorylation system

Hatefi, Y.; The Enzymes of Biological Membranes (Martonosi, A. V. , ed. ) Plenum, New York 4, 3-41 (1976)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.5.1
2,4-dinitrophenol
activation
Bos taurus
1.3.5.1
ATP
activation
Bos taurus
1.3.5.1
Chaotropic anions
activation
Bos taurus
1.3.5.1
FMNH2
activation
Bos taurus
1.3.5.1
fumarate
activation
Bos taurus
1.3.5.1
IDP
activation
Bos taurus
1.3.5.1
ITP
activation
Bos taurus
1.3.5.1
malonate
activation
Bos taurus
1.3.5.1
phosphate
activation
Bos taurus
1.3.5.1
Phospholipids
activation
Bos taurus
1.3.5.1
reduced ubiquinone-10
activation
Bos taurus
1.3.5.1
succinate
activation
Bos taurus
1.3.5.1
succinyl coenzyme A
activation
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
2-Thenoyltrifluoroacetone
-
Bos taurus
1.3.5.1
ferricyanide
at concentrations above 3 mM
Bos taurus
1.3.5.1
fumarate
-
Bos taurus
1.3.5.1
Maleate
-
Bos taurus
1.3.5.1
malonate
-
Bos taurus
1.3.5.1
Methylsuccinate
-
Bos taurus
1.3.5.1
oxaloacetate
competitive inhibitor
Bos taurus
1.3.5.1
thiol reagents
-
Bos taurus
7.1.1.2
barbiturates
-
Bos taurus
7.1.1.2
Demerol
-
Bos taurus
7.1.1.2
piercidin A
-
Bos taurus
7.1.1.2
rotenone
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.3
-
succinate
-
Bos taurus
7.1.1.2
0.007
-
NADH
reaction with ferricyanide
Bos taurus
7.1.1.2
0.012
-
cytochrome c
-
Bos taurus
7.1.1.2
0.014
-
NADH
reaction with cytochrome c
Bos taurus
7.1.1.2
4
-
ferricyanide
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.1.1.2
mitochondrion
-
Bos taurus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Fe
7-8 g-atoms of nonheme iron per 100000 g of protein
Bos taurus
7.1.1.2
Iron
contains 4 major iron centers, contains 16-18 gatoms of iron per 650000 Da enzyme
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.1
100000
-
succinate dehydrogenase
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.1
succinate + ubiquinone
Bos taurus
complex II of the mitochondrial oxidative phosphorylating system
fumarate + ubiquinol
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Bos taurus
-
succinate dehydrogenase
-
7.1.1.2
Bos taurus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.3.5.1
lipoprotein
-
Bos taurus
7.1.1.2
lipoprotein
the enzyme complex contains 0.22 mg of lipid per 650000 Da enzyme
Bos taurus
Purification (Commentary)
EC Number
Commentary
Organism
1.3.5.1
by selective resolution of complex II with chaotropic salts
Bos taurus
Renatured (Commentary)
EC Number
Commentary
Organism
1.3.5.1
reconstitution from partially purified succinate dehydrogenase, cytochrome b and phospholipids, reconstitution by the reversal of chaotrope-induced resolution of complex II
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
7.1.1.2
heart
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + ubiquinone
-
391082
Bos taurus
fumarate + ubiquinol
-
391082
Bos taurus
?
1.3.5.1
succinate + ubiquinone
complex II of the mitochondrial oxidative phosphorylating system
391082
Bos taurus
fumarate + ubiquinol
-
391082
Bos taurus
?
7.1.1.2
2 ferricyanide + NADH
activity is 27.4fold of the activity with ubiquinone-1
391082
Bos taurus
2 ferrocyanide + NAD+ + H+
-
-
-
?
7.1.1.2
2 ferricyanide + NADPH
0.13% of the activity with NADH and ferricyanide
391082
Bos taurus
2 ferrocyanide + NADP+ + H+
-
-
-
?
7.1.1.2
2 ferricytochrome c + NADH
12%-16% of the activity with ubiquinone-1
391082
Bos taurus
2 ferrocytochrome c + NAD+ + H+
-
-
-
?
7.1.1.2
NADH + 2-methylnaphthoquinone
7.6% of the activity with ubiquinone-1
391082
Bos taurus
NAD+ + ?
-
-
-
?
7.1.1.2
NADH + ubiquinone
-
391082
Bos taurus
NAD+ + ubiquinol
-
-
-
?
7.1.1.2
NADH + ubiquinone-1
-
391082
Bos taurus
NAD+ + ubiquinol-1
-
-
-
?
7.1.1.2
NADH + ubiquinone-6
86% of the activity with ubiquinone-1
391082
Bos taurus
NAD+ + ubiquinol-6
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
7.1.1.2
More
the enzyme complex is composed of at least 10 polypeptides ranging in MW from 10000 Da to 70000 Da
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
ATP
activation
Bos taurus
1.3.5.1
cytochrome b
4.5-5 nmol cytochrome b per mg protein; cytochrome b-557.5
Bos taurus
1.3.5.1
FAD
1 mol of covalently bound flavin per 100,000 g of protein; covalently bound to flavoprotein subunit
Bos taurus
1.3.5.1
fumarate
activation
Bos taurus
1.3.5.1
IDP
activation
Bos taurus
1.3.5.1
additional information
preparations of complex II contain 0.2 mg lipid per mg protein and 7-8 mol of acid-labile sulfide per 100,000 g of protein
Bos taurus
1.3.5.1
ubiquinone
-
Bos taurus
7.1.1.2
FMN
contains 1 FMN per 650000 Da enzyme
Bos taurus
7.1.1.2
NADH
-
Bos taurus
7.1.1.2
Ubiquinone-10
the enzyme contains 4.2-4.5 mol of ubiquinone-10 per 650000 Da enzyme
Bos taurus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.0015
-
oxaloacetate
-
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.5.1
2,4-dinitrophenol
activation
Bos taurus
1.3.5.1
ATP
activation
Bos taurus
1.3.5.1
Chaotropic anions
activation
Bos taurus
1.3.5.1
FMNH2
activation
Bos taurus
1.3.5.1
fumarate
activation
Bos taurus
1.3.5.1
IDP
activation
Bos taurus
1.3.5.1
ITP
activation
Bos taurus
1.3.5.1
malonate
activation
Bos taurus
1.3.5.1
phosphate
activation
Bos taurus
1.3.5.1
Phospholipids
activation
Bos taurus
1.3.5.1
reduced ubiquinone-10
activation
Bos taurus
1.3.5.1
succinate
activation
Bos taurus
1.3.5.1
succinyl coenzyme A
activation
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
ATP
activation
Bos taurus
1.3.5.1
cytochrome b
4.5-5 nmol cytochrome b per mg protein; cytochrome b-557.5
Bos taurus
1.3.5.1
FAD
1 mol of covalently bound flavin per 100,000 g of protein; covalently bound to flavoprotein subunit
Bos taurus
1.3.5.1
fumarate
activation
Bos taurus
1.3.5.1
IDP
activation
Bos taurus
1.3.5.1
additional information
preparations of complex II contain 0.2 mg lipid per mg protein and 7-8 mol of acid-labile sulfide per 100,000 g of protein
Bos taurus
1.3.5.1
ubiquinone
-
Bos taurus
7.1.1.2
FMN
contains 1 FMN per 650000 Da enzyme
Bos taurus
7.1.1.2
NADH
-
Bos taurus
7.1.1.2
Ubiquinone-10
the enzyme contains 4.2-4.5 mol of ubiquinone-10 per 650000 Da enzyme
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
2-Thenoyltrifluoroacetone
-
Bos taurus
1.3.5.1
ferricyanide
at concentrations above 3 mM
Bos taurus
1.3.5.1
fumarate
-
Bos taurus
1.3.5.1
Maleate
-
Bos taurus
1.3.5.1
malonate
-
Bos taurus
1.3.5.1
Methylsuccinate
-
Bos taurus
1.3.5.1
oxaloacetate
competitive inhibitor
Bos taurus
1.3.5.1
thiol reagents
-
Bos taurus
7.1.1.2
barbiturates
-
Bos taurus
7.1.1.2
Demerol
-
Bos taurus
7.1.1.2
piercidin A
-
Bos taurus
7.1.1.2
rotenone
-
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.0015
-
oxaloacetate
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.3
-
succinate
-
Bos taurus
7.1.1.2
0.007
-
NADH
reaction with ferricyanide
Bos taurus
7.1.1.2
0.012
-
cytochrome c
-
Bos taurus
7.1.1.2
0.014
-
NADH
reaction with cytochrome c
Bos taurus
7.1.1.2
4
-
ferricyanide
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.1.1.2
mitochondrion
-
Bos taurus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Fe
7-8 g-atoms of nonheme iron per 100000 g of protein
Bos taurus
7.1.1.2
Iron
contains 4 major iron centers, contains 16-18 gatoms of iron per 650000 Da enzyme
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.1
100000
-
succinate dehydrogenase
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.1
succinate + ubiquinone
Bos taurus
complex II of the mitochondrial oxidative phosphorylating system
fumarate + ubiquinol
-
Bos taurus
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.3.5.1
lipoprotein
-
Bos taurus
7.1.1.2
lipoprotein
the enzyme complex contains 0.22 mg of lipid per 650000 Da enzyme
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.1
by selective resolution of complex II with chaotropic salts
Bos taurus
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.1
reconstitution from partially purified succinate dehydrogenase, cytochrome b and phospholipids, reconstitution by the reversal of chaotrope-induced resolution of complex II
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
7.1.1.2
heart
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + ubiquinone
-
391082
Bos taurus
fumarate + ubiquinol
-
391082
Bos taurus
?
1.3.5.1
succinate + ubiquinone
complex II of the mitochondrial oxidative phosphorylating system
391082
Bos taurus
fumarate + ubiquinol
-
391082
Bos taurus
?
7.1.1.2
2 ferricyanide + NADH
activity is 27.4fold of the activity with ubiquinone-1
391082
Bos taurus
2 ferrocyanide + NAD+ + H+
-
-
-
?
7.1.1.2
2 ferricyanide + NADPH
0.13% of the activity with NADH and ferricyanide
391082
Bos taurus
2 ferrocyanide + NADP+ + H+
-
-
-
?
7.1.1.2
2 ferricytochrome c + NADH
12%-16% of the activity with ubiquinone-1
391082
Bos taurus
2 ferrocytochrome c + NAD+ + H+
-
-
-
?
7.1.1.2
NADH + 2-methylnaphthoquinone
7.6% of the activity with ubiquinone-1
391082
Bos taurus
NAD+ + ?
-
-
-
?
7.1.1.2
NADH + ubiquinone
-
391082
Bos taurus
NAD+ + ubiquinol
-
-
-
?
7.1.1.2
NADH + ubiquinone-1
-
391082
Bos taurus
NAD+ + ubiquinol-1
-
-
-
?
7.1.1.2
NADH + ubiquinone-6
86% of the activity with ubiquinone-1
391082
Bos taurus
NAD+ + ubiquinol-6
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.1.1.2
More
the enzyme complex is composed of at least 10 polypeptides ranging in MW from 10000 Da to 70000 Da
Bos taurus