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Literature summary extracted from

  • Hatefi, Y.
    The enzymes and the enzyme complexes of the mitochondrial oxidative phosphorylation system (1976), The Enzymes of Biological Membranes (Martonosi, A. V. , ed. ) Plenum, New York, 4, 3-41.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.5.1 2,4-dinitrophenol activation Bos taurus
1.3.5.1 ATP activation Bos taurus
1.3.5.1 Chaotropic anions activation Bos taurus
1.3.5.1 FMNH2 activation Bos taurus
1.3.5.1 fumarate activation Bos taurus
1.3.5.1 IDP activation Bos taurus
1.3.5.1 ITP activation Bos taurus
1.3.5.1 malonate activation Bos taurus
1.3.5.1 phosphate activation Bos taurus
1.3.5.1 Phospholipids activation Bos taurus
1.3.5.1 reduced ubiquinone-10 activation Bos taurus
1.3.5.1 succinate activation Bos taurus
1.3.5.1 succinyl coenzyme A activation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 2-Thenoyltrifluoroacetone
-
 Bos taurus
1.3.5.1 ferricyanide at concentrations above 3 mM Bos taurus
1.3.5.1 fumarate
-
 Bos taurus
1.3.5.1 Maleate
-
 Bos taurus
1.3.5.1 malonate
-
 Bos taurus
1.3.5.1 Methylsuccinate
-
 Bos taurus
1.3.5.1 oxaloacetate competitive inhibitor Bos taurus
1.3.5.1 thiol reagents
-
 Bos taurus
7.1.1.2 barbiturates
-
 Bos taurus
7.1.1.2 Demerol
-
 Bos taurus
7.1.1.2 piercidin A
-
 Bos taurus
7.1.1.2 rotenone
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.3
-
 succinate
-
 Bos taurus
7.1.1.2 0.007
-
 NADH reaction with ferricyanide Bos taurus
7.1.1.2 0.012
-
 ferricytochrome c
-
 Bos taurus
7.1.1.2 0.014
-
 NADH reaction with cytochrome c Bos taurus
7.1.1.2 4
-
 ferricyanide
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.5.1 Fe 7-8 g-atoms of nonheme iron per 100000 g of protein Bos taurus
7.1.1.2 Iron contains 4 major iron centers, contains 16-18 gatoms of iron per 650000 Da enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.1 100000
-
 succinate dehydrogenase Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.1 succinate + ubiquinone Bos taurus complex II of the mitochondrial oxidative phosphorylating system fumarate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Bos taurus
-
 succinate dehydrogenase
-
7.1.1.2 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.3.5.1 lipoprotein
-
 Bos taurus
7.1.1.2 lipoprotein the enzyme complex contains 0.22 mg of lipid per 650000 Da enzyme Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.1 by selective resolution of complex II with chaotropic salts Bos taurus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.3.5.1 reconstitution from partially purified succinate dehydrogenase, cytochrome b and phospholipids, reconstitution by the reversal of chaotrope-induced resolution of complex II Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.2 heart
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 succinate + ubiquinone
-
 Bos taurus fumarate + ubiquinol
-
 ?
1.3.5.1 succinate + ubiquinone complex II of the mitochondrial oxidative phosphorylating system Bos taurus fumarate + ubiquinol
-
 ?
7.1.1.2 2 ferricyanide + NADH activity is 27.4fold of the activity with ubiquinone-1 Bos taurus 2 ferrocyanide + NAD+ + H+
-
 ?
7.1.1.2 2 ferricyanide + NADPH 0.13% of the activity with NADH and ferricyanide Bos taurus 2 ferrocyanide + NADP+ + H+
-
 ?
7.1.1.2 2 ferricytochrome c + NADH 12%-16% of the activity with ubiquinone-1 Bos taurus 2 ferrocytochrome c + NAD+ + H+
-
 ?
7.1.1.2 NADH + 2-methylnaphthoquinone 7.6% of the activity with ubiquinone-1 Bos taurus NAD+ + 2-methylnaphthalene-1,4-diol
-
 ?
7.1.1.2 NADH + ferricytochrome c
-
 Bos taurus NAD+ + H+ + ferrocytochrome c
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Bos taurus NAD+ + ubiquinol
-
 ?
7.1.1.2 NADH + ubiquinone-1
-
 Bos taurus NAD+ + ubiquinol-1
-
 ?
7.1.1.2 NADH + ubiquinone-6 86% of the activity with ubiquinone-1 Bos taurus NAD+ + ubiquinol-6
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.2 More the enzyme complex is composed of at least 10 polypeptides ranging in MW from 10000 Da to 70000 Da Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 ATP activation Bos taurus
1.3.5.1 cytochrome b 4.5-5 nmol cytochrome b per mg protein Bos taurus
1.3.5.1 cytochrome b cytochrome b-557.5 Bos taurus
1.3.5.1 FAD covalently bound to flavoprotein subunit Bos taurus
1.3.5.1 FAD 1 mol of covalently bound flavin per 100,000 g of protein Bos taurus
1.3.5.1 fumarate activation Bos taurus
1.3.5.1 IDP activation Bos taurus
1.3.5.1 additional information preparations of complex II contain 0.2 mg lipid per mg protein and 7-8 mol of acid-labile sulfide per 100,000 g of protein Bos taurus
1.3.5.1 ubiquinone
-
 Bos taurus
7.1.1.2 FMN contains 1 FMN per 650000 Da enzyme Bos taurus
7.1.1.2 NADH
-
 Bos taurus
7.1.1.2 Ubiquinone-10 the enzyme contains 4.2-4.5 mol of ubiquinone-10 per 650000 Da enzyme Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.1 0.0015
-
 oxaloacetate
-
 Bos taurus